Metabolic engineering strategies to enable microbial electrosynthesis utilization of CO: recent progress and challenges.

Crit Rev Biotechnol

Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, China.

Published: May 2024

Microbial electrosynthesis (MES) is a promising technology that mainly utilizes microbial cells to convert CO into value-added chemicals using electrons provided by the cathode. However, the low electron transfer rate is a solid bottleneck hindering the further application of MES. Thus, as an effective strategy, genetic tools play a key role in MES for enhancing the electron transfer rate and diversity of production. We describe a set of genetic strategies based on fundamental characteristics and current successes and discuss their functional mechanisms in driving microbial electrocatalytic reactions to fully comprehend the roles and uses of genetic tools in MES. This paper also analyzes the process of nanomaterial application in extracellular electron transfer (EET). It provides a technique that combines nanomaterials and genetic tools to increase MES efficiency, because nanoparticles have a role in the production of functional genes in EET although genetic tools can subvert MES, it still has issues with difficult transformation and low expression levels. Genetic tools remain one of the most promising future strategies for advancing the MES process despite these challenges.

Download full-text PDF

Source
http://dx.doi.org/10.1080/07388551.2023.2167065DOI Listing

Publication Analysis

Top Keywords

genetic tools
20
electron transfer
12
microbial electrosynthesis
8
transfer rate
8
mes
7
genetic
6
tools
5
metabolic engineering
4
engineering strategies
4
strategies enable
4

Similar Publications

Despite all the progress in treating SARS-CoV-2, escape mutants to current therapies remain a constant concern. Promising alternative treatments for current and future coronaviruses are those that limit escape mutants by inhibiting multiple pathogenic targets, analogous to the current strategies for treating HCV and HIV. With increasing popularity and ease of manufacturing of RNA technologies for vaccines and drugs, therapeutic microRNAs represent a promising option.

View Article and Find Full Text PDF

The increasingly widespread application of next-generation sequencing (NGS) in clinical diagnostics and epidemiological research has generated a demand for robust, fast, automated, and user-friendly bioinformatics workflows. To guide the choice of tools for the assembly of full-length viral genomes from NGS datasets, we assessed the performance and applicability of four open-source bioinformatics pipelines (shiver-for which we created a user-friendly Dockerized version, referred to as dshiver; SmaltAlign; viral-ngs; and V-pipe) using both simulated and real-world HIV-1 paired-end short-read datasets and default settings. All four pipelines produced consensus genome assemblies with high quality metrics (genome fraction recovery, mismatch and indel rates, variant calling F1 scores) when the reference sequence used for assembly had high similarity to the analyzed sample.

View Article and Find Full Text PDF

Malaria poses a serious global health problem, with half the world population being at risk. Regular screening is crucial for breaking the transmission cycle and combatting the disease spreading. However, current diagnostic tools relying on blood samples face challenges in many malaria-epidemic areas.

View Article and Find Full Text PDF

Bladder cancer is one of the most common cancers worldwide. Despite its high incidence, cystoscopy remains the currently used diagnostic gold standard, although it is invasive, expensive and has low sensitivity. As a result, the cancer diagnosis is mostly late, as it occurs following the presence of hematuria in urine, and population screening is not allowed.

View Article and Find Full Text PDF

Mining of Candidate Genes and Developing Molecular Markers Associated with Pokkah Boeng Resistance in Sugarcane ( spp.).

Plants (Basel)

December 2024

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530005, China.

Sugarcane Pokkah Boeng (PB), a fungal disease caused by spp., poses a significant threat to sugar industries globally. Breeding sugarcane varieties resistant to PB has become a priority, and the mining of PB resistance genes and the development of molecular markers provide a solid foundation for this purpose.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!