AI Article Synopsis
- Zinc finger nucleases (ZFNs) previously played a vital role in genome editing but are now less common due to newer technologies like TALENs and CRISPR-Cas that are simpler and less labor-intensive.
- Despite their decline, expired foundational ZFN patents allow for broader use in clinical and industrial settings.
- The article presents a new protocol for constructing ZFNs using bacterial one-hybrid selection and a single-strand annealing assay.
Article Abstract
Zinc finger nucleases (ZFNs) are programmable nucleases that have contributed significantly to past genome-editing research. They are now utilized much less owing to the advent of transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats and CRISPR-associated protein system (CRISPR-Cas). These new methods allow for easier generation of reagents that target genomic sequences of interest and are less labor-intensive than ZFNs at targeting desired sequences. However, fundamental ZFN patents have expired, enabling a wide range of their distribution for clinical and industrial applications. This article introduces a ZFN construction protocol that uses bacterial one-hybrid (B1H) selection and single-strand annealing (SSA) assay.
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| http://dx.doi.org/10.1007/978-1-0716-3016-7_1 | DOI Listing |
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