The effect of heparin treatment on clathrin basketwork of cell membranes was examined in cultured cells derived from the stromal-vascular fraction of neonatal mouse brown adipose tissue. The cytoplasmic surface of the plasma membrane of ruptured cells was examined with surface replicas. Heparin treatment significantly decreased the extent of clathrin basketwork associated with the membrane from 3.4 to 0.2%. The loss of clathrin basketwork suggests that heparin treatment of these cells affected intracellular sorting processes associated with endocytosis.
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Effect of heparin on membrane associated clathrin basketwork of cultured cells derived from the stromal-vascular fraction of mouse brown adipose tissue.
Cell Biol Int Rep
September 1987
Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
The effect of heparin treatment on clathrin basketwork of cell membranes was examined in cultured cells derived from the stromal-vascular fraction of neonatal mouse brown adipose tissue. The cytoplasmic surface of the plasma membrane of ruptured cells was examined with surface replicas. Heparin treatment significantly decreased the extent of clathrin basketwork associated with the membrane from 3.
View Article and Find Full Text PDFIsolated Golgi membranes incubated in the presence of ATP and a cytosolic protein fraction form a population of coated buds or vesicles from the Golgi cisternae. The coats do not have the characteristic hexagonal-pentagonal basketwork of clathrin, and do not react with anti-clathrin polyclonal antibody. The conditions that produce these apparently nonclathrin-coated buds also reconstitute protein transport between compartments of the Golgi stack.
View Article and Find Full Text PDFFixation of HeLa cells with a mixture of 100 mM glutaraldehyde, 2 mg/ml tannic acid and 0.5 mg/ml saponin allows the tannic acid to penetrate intact cells without disruption of membranes or extraction of the cytoplasmic matrix. After subsequent treatment with OsO4 cytoplasmic structures are stained so densely that fine details are visible even in very thin (dark gray) sections.
View Article and Find Full Text PDFThe initial events during phagocytosis of latex beads by mouse peritoneal macrophages were visualized by high-resolution electron microscopy of platinum replicas of freeze-dried cells and by conventional thin-section electron microscopy of macrophages postfixed with 1% tannic acid. On the external surface of phagocytosing macrophages, all stages of particle uptake were seen, from early attachment to complete engulfment. Wherever the plasma membrane approached the bead surface, there was a 20-nm-wide gap bridged by narrow strands of material 12.
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