CLE peptides are well-known hormonal regulators of plant development, but their role in somatic embryogenesis remains undetermined. genes are often regulated by WOX transcription factors and, in their turn, regulate the expression level of genes. In this study, we used in vitro cultivation, as well as qPCR and transcriptomic analysis, to find CLE peptides which could regulate the gene, stimulating somatic embryogenesis in . Three CLE peptides were found which could probably be such regulators, but none of them was found to influence expression in the embryogenic calli. Nevertheless, overexpression of one of genes under study, , decreased somatic embryogenesis intensity. Additionally, overexpression of was found to suppress expression of , a supposed antagonist of somatic embryo development. Our findings can be helpful for the search for new regeneration regulators which could be used for plant transformation.
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| http://dx.doi.org/10.3390/plants12030435 | DOI Listing |
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