Biomimetic apatites exhibit a high reactivity allowing ion substitutions to modulate their in vivo response. We developed a novel approach combining several bioactive ions in a spatially controlled way in view of subsequent releases to address the sequence of events occurring after implantation, including potential microorganisms' colonization. Innovative micron-sized core-shell particles were designed with an external shell enriched with an antibacterial ion and an internal core substituted with a pro-angiogenic or osteogenic ion. After developing the proof of concept, two ions were particularly considered, Ag in the outer shell and Cu in the inner core. In vitro evaluations confirmed the cytocompatibility through Ag-/Cu-substituting and the antibacterial properties provided by Ag. Then, these multifunctional "smart" particles were embedded in a polymeric matrix by freeze-casting to prepare 3D porous scaffolds for bone engineering. This approach envisions the development of a new generation of scaffolds with tailored sequential properties for optimal bone regeneration.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9919144 | PMC |
http://dx.doi.org/10.3390/nano13030519 | DOI Listing |
Genes Dev
December 2024
Howard Hughes Medical Institute, Department of Biological Sciences, Columbia University, New York, New York 10025, USA.
The pan-neuronally expressed and phylogenetically conserved CUT homeobox gene orchestrates pan-neuronal gene expression throughout the nervous system of As in many other species, including humans, is encoded by a complex locus that also codes for a Golgi-localized protein, called CASP (Cux1 alternatively spliced product) in humans and CONE-1 ("CASP of nematodes") in How gene expression from this complex locus is controlled-and, in , directed to all cells of the nervous system-has not been investigated. We show here that pan-neuronal expression of CEH-44/CUX is controlled by a pan-neuronal RNA splicing factor, UNC-75, the homolog of vertebrate CELF proteins. During embryogenesis, the locus exclusively produces the Golgi-localized CONE-1/CASP protein in all tissues, but upon the onset of postmitotic terminal differentiation of neurons, UNC-75/CELF induces the production of the alternative CEH-44/CUX CUT homeobox gene-encoding transcript exclusively in the nervous system.
View Article and Find Full Text PDFNanomaterials (Basel)
December 2024
Personalized Medicine Centre, Almazov National Medical Research Centre, Akkuratova Str. 2, 197341 St. Petersburg, Russia.
This study addresses issues in developing spatially controlled magnetic fields for particle guidance, synthesizing biocompatible and chemically stable MNPs and enhancing their specificity to pathological cells through chemical modifications, developing personalized adjustments, and highlighting the potential of tumor-on-a-chip systems, which can simulate tissue environments and assess drug efficacy and dosage in a controlled setting. The research focused on two MNP types, uncoated magnetite nanoparticles (mMNPs) and carboxymethyl dextran coated superparamagnetic nanoparticles (CD-SPIONs), and evaluated their transport properties in microfluidic systems and porous media. The original uncoated mMNPs of bimodal size distribution and the narrow size distribution of the fractions (23 nm and 106 nm by radii) were demonstrated to agglomerate in magnetically driven microfluidic flow, forming a stable stationary web consisting of magnetic fibers within 30 min.
View Article and Find Full Text PDFCurr Issues Mol Biol
December 2024
Department of Cell Biology and Physiology, Brigham Young University, 3054 Life Sciences Building, Provo, UT 84602, USA.
Receptors for advanced glycation end products (RAGE) are multiligand cell surface receptors found most abundantly in lung tissue. This study sought to evaluate the role of RAGE in lung development by using a transgenic (TG) mouse model that spatially and temporally controlled RAGE overexpression. Histological imaging revealed that RAGE upregulation from embryonic day (E) 15.
View Article and Find Full Text PDFMethods Mol Biol
December 2024
University of Münster Institute of Physiological Chemistry and Pathobiochemistry, Münster, Germany.
The precise spatial and temporal regulation of cell-cell adhesions is crucial for understanding the underlying biological processes and for assembling multicellular structures in tissue engineering. Traditional approaches have relied on chemical membrane functionalization and regulated gene expression of native cell adhesion molecules (CAMs), but these methods lack the necessary control and can be detrimental to cells. In contrast, engineered photoswitchable cell-cell adhesions offer a reversible and dynamic regulation at a single-cell resolution.
View Article and Find Full Text PDFMethods Mol Biol
December 2024
Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Gram-negative bacteria can use the type III secretion system (T3SS) to inject effector proteins into eukaryotic target cells. In this chapter, we describe the application of a light-controlled T3SS, based on the targeted sequestration of an essential dynamic T3SS component with the help of optogenetic interaction switches. This method enables to control the secretion or injection into eukaryotic cells for a wide range of protein cargos with high temporal and spatial precision.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!