N6-methyladenosine (mA) is a post-transcriptional modification of RNA involved in transcript transport, degradation, translation, and splicing. We found that HBV RNA is modified by mA predominantly in the coding region of . The mutagenesis of methylation sites reduced the HBV mRNA and HBs protein levels. The suppression of mA by an inhibitor or knockdown in primary hepatocytes decreased the viral RNA and HBs protein levels in the medium. These results suggest that the mA modification of HBV RNA is needed for the efficient replication of HBV in hepatocytes.
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| http://dx.doi.org/10.3390/ijms24032265 | DOI Listing |
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