Cholangiocytes play a crucial role in bile formation. Cholangiocyte injury causes cholestasis, including primary biliary cholangitis (PBC). However, the etiology of PBC remains unclear despite being characterized as an autoimmune disease. Using single-cell RNA sequencing (scRNA-seq), fluorescence-activated-cell-sorting, multiplex immunofluorescence (IF) and RNAscope analyses, we identified unique DUOX2ACE2 small cholangiocytes in human and mouse livers. Their selective decrease in PBC patients was associated with the severity of disease. Moreover, proteomics, scRNA-seq, and qPCR analyses indicated that polymeric immunoglobulin receptor (pIgR) was highly expressed in DUOX2ACE2 cholangiocytes. Serum anti-pIgR autoantibody levels were significantly increased in PBC patients, regardless of positive and negative AMA-M2. Spatial transcriptomics and multiplex IF revealed that CD27 memory B and plasma cells accumulated in the hepatic portal tracts of PBC patients. Collectively, DUOX2ACE2 small cholangiocytes are pathogenic targets in PBC, and preservation of DUOX2ACE2 cholangiocytes and targeting anti-pIgR autoantibodies may be valuable strategies for therapeutic interventions in PBC.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9911648 | PMC |
http://dx.doi.org/10.1038/s41467-022-34606-w | DOI Listing |
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