Visualizing intracellular target antigens in live cells.

Trends Cell Biol

Disease Intervention Technology Laboratory, Institute of Molecular and Cellular Biology, Agency for Science Technology and Research (A*STAR), Singapore, Singapore. Electronic address:

Published: April 2023

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Article Abstract

In order to further visualize intracellular dynamics, precise imaging of endogenous proteins in live cells was performed using an antigen-binding fragment (Fab)-based Quenchbody (Q-body). The transfected Q-body probe showed an antigen-dependent fluorescence response, enabling the clear visualization and sorting of cells expressing p53, a tumor suppressor biomarker.

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http://dx.doi.org/10.1016/j.tcb.2023.01.004DOI Listing

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