AI Article Synopsis
- This study focused on creating a hybrid-glycolysis yeast, which is unique since glycolysis is the primary glucose breakdown pathway in eukaryotes.
- Researchers found that the OCA5 gene encodes an inositol pyrophosphatase that helps regulate glycolysis and respiration by controlling specific signaling molecules (5-InsP) tied to ATP levels.
- The hybrid-glycolysis yeast showed promising results by not producing ethanol when excess glucose was present and achieving a record production of free fatty acids (2.68 g/L), highlighting the potential of this yeast in metabolic regulation.
Article Abstract
Although many prokaryotes have glycolysis alternatives, it's considered as the only energy-generating glucose catabolic pathway in eukaryotes. Here, we managed to create a hybrid-glycolysis yeast. Subsequently, we identified an inositol pyrophosphatase encoded by OCA5 that could regulate glycolysis and respiration by adjusting 5-diphosphoinositol 1,2,3,4,6-pentakisphosphate (5-InsP) levels. 5-InsP levels could regulate the expression of genes involved in glycolysis and respiration, representing a global mechanism that could sense ATP levels and regulate central carbon metabolism. The hybrid-glycolysis yeast did not produce ethanol during growth under excess glucose and could produce 2.68 g/L free fatty acids, which is the highest reported production in shake flask of Saccharomyces cerevisiae. This study demonstrated the significance of hybrid-glycolysis yeast and determined Oca5 as an inositol pyrophosphatase controlling the balance between glycolysis and respiration, which may shed light on the role of inositol pyrophosphates in regulating eukaryotic metabolism.
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| http://dx.doi.org/10.1016/j.cell.2023.01.014 | DOI Listing |
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