AI Article Synopsis
- - The study investigates how tRNA-derived fragments, specifically mt-Ty 5' tiRNAs, influence gene expression in skeletal muscle cell growth and development.
- - Techniques like Northern blotting, RT-PCR, and RNA-Seq were used to analyze the impact of altering mt-Ty 5' tiRNA levels on muscle cell proliferation and differentiation.
- - Findings revealed that lower levels of mt-Ty 5' tiRNAs hinder muscle cell growth and development, while increasing these fragments promotes healthier muscle cell function, suggesting their potential as new targets for treating muscle diseases.
Article Abstract
In this study, we sought to determine the role of tRNA-derived fragments in the regulation of gene expression during skeletal muscle cell proliferation and differentiation. We employed cell culture to examine the function of mt-Ty 5' tiRNAs. Northern blotting, RT-PCR as well as RNA-Seq, were performed to determine the effects of mt-Ty 5' tiRNA loss and gain on gene expression. Standard and transmission electron microscopy (TEM) were used to characterize cell and sub-cellular structures. mt-Ty 5'tiRNAs were found to be enriched in mouse skeletal muscle, showing increased levels in later developmental stages. Gapmer-mediated inhibition of tiRNAs in skeletal muscle C2C12 myoblasts resulted in decreased cell proliferation and myogenic differentiation; consistent with this observation, RNA-Seq, transcriptome analyses, and RT-PCR revealed that skeletal muscle cell differentiation and cell proliferation pathways were also downregulated. Conversely, overexpression of mt-Ty 5'tiRNAs in C2C12 cells led to a reversal of these transcriptional trends. These data reveal that mt-Ty 5'tiRNAs are enriched in skeletal muscle and play an important role in myoblast proliferation and differentiation. Our study also highlights the potential for the development of tiRNAs as novel therapeutic targets for muscle-related diseases.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10392060 | PMC |
| http://dx.doi.org/10.1111/cpr.13416 | DOI Listing |
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