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Investigation into the effect of phenylalanine gating on anaerobic haem breakdown using the energy landscape approach.
Protein Sci
February 2025
Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, UK.
We have recently demonstrated a novel anaerobic NADH-dependent haem breakdown reaction, which is carried out by a range of haemoproteins. The Yersinia enterocolitica protein, HemS, is the focus of further research presented in the current paper. Using conventional experimental methods, bioinformatics, and energy landscape theory (ELT), we provide new insight into the mechanism of the novel breakdown process.
View Article and Find Full Text PDFThyme Essential Oil as a Potential Tool Against Common and Re-Emerging Foodborne Pathogens: Biocidal Effect on Bacterial Membrane Permeability.
Microorganisms
December 2024
Department of Molecular Medicine and Medical Biotechnologies, University of Naples "Federico II", 80131 Naples, Italy.
Over the past decade, foodborne diseases have become a significant public health concern, affecting millions of people globally. Major pathogens like spp., , , and contaminate food and cause several infections.
View Article and Find Full Text PDFThe non-nutritive sweetener rebaudioside a enhances phage infectivity.
Sci Rep
January 2025
Department of Nutritional Biochemistry, University of Hohenheim, Garbenstraße 30, 70599, Stuttgart, Germany.
Non-nutritive sweeteners (NNS) are widely employed in foodstuffs. However, it has become increasingly evident that their consumption is associated with bacterial dysbiosis, which, in turn, is linked to several health conditions, including a higher risk of type 2 diabetes and cancer. Among the NNS, stevia, whose main component is rebaudioside A (rebA), is gaining popularity in the organic food market segment.
View Article and Find Full Text PDFApproaches for the Inactivation of .
Appl Biosaf
December 2024
Department of Microbiology and Immunology; University of Louisville School of Medicine, Louisville, Kentucky, USA.
Introduction: is the gram-negative, facultative intracellular bacterium that causes the disease known as plague. Due to the risk for aerosol transmission, a low infectious dose, and the acute and lethal nature of pneumonic plague, research activities with require Biosafety Level 3 (BSL-3) facilities to provide the appropriate safeguards to minimize accidental exposures and environmental release. However, many experimental assays cannot be performed in BSL-3 due to equipment availability, and thus require removal of samples from the BSL-3 laboratory to be completed.
View Article and Find Full Text PDFBehaviour of Listeria monocytogenes and Yersinia enterocolitica during beef dry-aging up to 60 days.
Int J Food Microbiol
February 2025
Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Italy.
The Commission delegated Regulation (EU) 2024/1141 states that beef dry aging can be performed in a dedicated cabinet, at a surface temperature of -0.5 to 3 °C, maximum relative humidity of 85 %, air speed of 0.2-0.
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