Background: Herbicide resistance is one of the threats to modern agriculture and its early detection is one of the most effective components for sustainable resistance management strategies. Many techniques have been used for target-site-resistance detection. Allele-Specific Loop-Mediated Isothermal Amplification (AS-LAMP) was evaluated as a possible rapid diagnostic method for acetyl-CoA carboxylase (ACCase) and acetolactate synthase (ALS) inhibiting herbicides resistance in Lolium spp.
Results: AS-LAMP protocols were set up for the most frequent mutations responsible for herbicide resistance to ALS (positions 197, 376 and 574) and ACCase (positions 1781, 2041 and 2078) inhibitors in previously characterized and genotyped Lolium spp.
Populations: A validation step on new putative resistant populations gave the overview of a possible use of this tool for herbicide resistance diagnosis in Lolium spp. Regarding the ACCase inhibitor pinoxaden, in more than 65% of the analysed plants, the LAMP assay and genotyping were in keeping, whereas the results were not consistent when ALS inhibitors resistance was considered. Limitations on the use of this technique for herbicide resistance detection in the allogamous Lolium spp. are discussed.
Conclusions: The LAMP method used for the detection of target-site resistance in weed species could be applicable with target genes that do not have high genetic variability, such as ACCase gene in Lolium spp.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9906911 | PMC |
| http://dx.doi.org/10.1186/s13007-023-00989-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Investigating non-target site resistance to pyroxsulam in a glyphosate-resistant Lolium rigidum population.
Pest Manag Sci
January 2025
College of Plant Protection, Hunan Agricultural University, Changsha, China.
Background: Resistance to multiple herbicides is common in Lolium rigidum. Here, resistance to acetolactate synthase (ALS)- and susceptibility to acetyl-CoA carboxylase (ACCase)-inhibiting herbicides was confirmed in a glyphosate-resistant L. rigidum population (NLR70) from Australia and the mechanisms of pyroxsulam resistance were examined.
View Article and Find Full Text PDFAffecting of Glyphosate Tolerance and Metabolite Content in Transgenic Overexpressing Gene from .
Plants (Basel)
December 2024
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Long-term use of the global non-selective herbicide glyphosate for weed control has caused resistance in weeds. Overproducing of the target of glyphosate 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) is one of the resistance mechanisms in weeds. However, few studies have measured the effects on tolerance levels and metabolite content in model plant species overexpressing from weeds.
View Article and Find Full Text PDFPyroxsulam Resistance in : An Emerging Challenge in Crop Protection.
Plants (Basel)
December 2024
Department of Agroecology and Crop Production, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Prague, Czech Republic.
, a prevalent weed in Czech winter wheat fields, has developed resistance to ALS-inhibiting herbicides due to their frequent use. This study reports a biotype of resistant to pyroxsulam, with cross and multiple resistance to iodosulfuron, propoxycarbazone, pinoxaden, and chlortoluron. Dose-response experiments revealed high resistance of both R1 and R2 biotypes to pyroxsulam, with resistance factors (RF) of 6.
View Article and Find Full Text PDFHerbicide and irrigation management options in conventionally-tilled wheat: deciphering water and energy budgeting, and grain and monetary output in north-Indian plains.
Sci Rep
January 2025
Institute of Agricultural Sciences, Banaras Hindu University, India, Varanasi.
In South Asia, declining water tables due to increased irrigation and labor shortages for manual weeding pose significant challenges for wheat production. Additionally, herbicide resistance, often resulting from poor management practices, further complicates weed problems. The objective of this study was to assess the impacts of traditional irrigation regimens (IRs) and herbicide application on wheat crops.
View Article and Find Full Text PDFExpression of Agrobacterium Isopentenyl transferase (IPT) gene in wheat improves drought tolerance.
Transgenic Res
January 2025
Kauser Abdulla Malik School of Life Sciences, Forman Christian College (A Chartered University), Ferozpur Road, Lahore, 54600, Pakistan.
Drought, as an abiotic stressor, globally limits cereal productivity, leading to early aging of leaves and lower yields. The expression of the isopentenyl transferase (IPT) gene, which is involved in cytokinin (CK) biosynthesis, can delay drought-induced leaf senescence. In this study, the Agrobacterium Isopentenyl transferase (IPT) gene was introduced into two local hexaploid wheat cultivars, NR-421 and FSD-2008.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!