sp. nov., isolated from landfill leachate.

Int J Syst Evol Microbiol

College of Civil Engineering and Architecture, Zhejiang University, Hangzhou 310058, PR China.

Published: February 2023

Bacterial strain Y-6, isolated from a landfill site in Yiwu, PR China, was characterized using a polyphasic taxonomy approach. Cells were Gram-stain-negative, aerobic, rod-shaped, motile by means of a single polar flagellum and formed pale beige colonies. Strain Y-6 grew at 4-40 °C (optimal at 30-37 °C), pH 6.5-9.5 (optimal at pH 7.2-8.5) and in the presence of 0.5-10.0 % (w/v) NaCl (optimal at 1.0-3.0 %). Phylogenetic analysis revealed that strain Y-6 was a member of the genus and closely related to MCCC 1A06493 with a 16S rRNA sequence similarity of 98.2 %. The major cellular fatty acids of the isolate were iso-C, C, iso-C and summed feature 9 (iso-C 9 and/or 10-methyl-C). Q-8 was the predominant ubiquinone. The major polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, aminoglycophospholipid, aminophospholipid, phospholipid, three glycolipids and two unknown lipids. The genomic DNA G+C content was 46.6 mol%. The digital DNA-DNA hybridization value between Y-6 and A. taiwanensis MCCC 1A06493 was 18.3 %. Strain Y-6 had an average nucleotide identity value of 74.09 % with A. taiwanensis MCCC 1A06493. Results from the polyphasic taxonomy study support the conclusion that strain Y-6 represents a novel species, for which the name sp.nov. is proposed. The type strain is Y-6 (=MCCC 1K06228=KCTC 82676).

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http://dx.doi.org/10.1099/ijsem.0.005685DOI Listing

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