A novel bacterial strain, CBA7301, was isolated from human faeces and was characterised using a polyphasic taxonomic approach. A phylogenetic analysis based on 16S rRNA gene sequences revealed that CBA7301 represented a member of the genus , in the family . The similarity between the 16S rRNA gene sequence of CBA7301 and that of its most closely related species, JCM 17102, was 96.2 %, and the average nucleotide identity between these two strains was 77.9 %. The genome size was 6 782 182 bp, and the DNA G+C content was 42.5 mol%. Cells of CBA7301 were Gram-stain-negative, strictly anaerobic and rod-shaped. The optimal growth of this organism occurred at 30-35 °C, pH 7.0 and 0.5 % (w/v) NaCl. The respiratory quinone was menaquinone 10. The predominant polar lipids were phosphatidylethanolamine, phospholipids and aminophospholipids. The major cellular fatty acid was anteiso-C. According to the results of the polyphasic taxonomic analysis, CBA7301 represents a novel species of the genus , which we named sp. nov. The type strain is CBA7301 (=KCCM 43355=ATCC TSD-227).
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http://dx.doi.org/10.1099/ijsem.0.005666 | DOI Listing |
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