Objective: The objective of this study was to screen lymphoma radiotherapy-resistant genes using CRISPR activation (CRISPRa).
Methods: The Human CRISPRa library virus was packaged and then transfected into lymphoma cells to construct an activation library cell line, which was irradiated at the minimum lethal radiation dose to screen radiotherapy-resistant cells. Radiotherapy-resistant cell single-guide RNA (sgRNA) was first amplified by quantitative polymerase chain reaction (qPCR) in the coding region and then subject to next-generation sequencing (NGS) and bioinformatics analysis to screen radiotherapy-resistant genes. Certain radiotherapy-resistant genes were then selected to construct activated cell lines transfected with a single gene so as to further verify the relationship between gene expression and radiotherapy resistance.
Results: A total of 16 radiotherapy-resistant genes, namely, , and , were screened based on the NGS results and bioinformatics analysis of the radiotherapy-resistant cells. Activated cell lines transfected with a single gene were constructed using 10 radiotherapy-resistant genes. The qPCR findings showed that, when compared with the control group, the experimental group had significantly up-regulated mRNA expression of , and (p < 0.05). No significant difference in the mRNA expression of or (p > 0.05) was found between the two groups (p > 0.05).
Conclusion: The 16 genes screened are potential lymphoma radiotherapy-resistant genes. It was initially determined that the high expression of 8 genes was associated with lymphoma radiotherapy resistance, and these genes could serve as the potential biomarkers for predicting lymphoma radiotherapy resistance or as new targets for therapy.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9897072 | PMC |
| http://dx.doi.org/10.2147/PGPM.S386085 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Analysis of the Mechanism Underlying Radiotherapy Resistance Caused by Oligodendroglia Cells in Glioblastoma by Applying the Single-cell RNA Sequencing Technology.
Curr Med Chem
September 2024
Neurosurgery, Daqing Oil Field General Hospital, Daqing, 163000, China.
Background: Glioblastoma (GBM) is an aggressive malignancy. The inherent resistance of GBM to radiotherapy poses great challenges for clinical treatment.
Objectives: The primary objective of this study is to explore the molecular mechanisms of radiotherapy resistance in GBM and identify the key influencing factors that contribute to this phenomenon.
NSUN6-mediated 5-methylcytosine modification of NDRG1 mRNA promotes radioresistance in cervical cancer.
Mol Cancer
July 2024
Department of Gynecologic Oncology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.
Background: Radioresistance is the leading cause of death in advanced cervical cancer (CC). Dysregulation of RNA modification has recently emerged as a regulatory mechanism in radiation and drug resistance. We aimed to explore the biological function and clinical significance of 5-methylcytosine (mC) in cervical cancer radiosensitivity.
View Article and Find Full Text PDF(-) - Epicatechin regulates LOC107986454 by targeting the miR-143-3p/EZH2 axis to enhance the radiosensitivity of non-small cell lung cancer.
Am J Med Sci
November 2024
Department of Hepatobiliary Surgery, The First People's Hospital of Qujing, The Qujing Affiliated Hospital of Kunming Medical University, Qujing, Yunnan, 655000, China. Electronic address:
Background And Objective: Non-small cell lung cancer (NSCLC) is a pernicious tumor with high incidence and mortality rates. The incidence rate of NSCLC increases with age and poses a serious danger to human health. The aim of this study was to determine the mechanism by which (-)-epicatechin (EC) alleviates NSCLC.
View Article and Find Full Text PDFMogrol-mediated enhancement of radiotherapy sensitivity in non-small cell lung cancer: a mechanistic study.
Am J Physiol Cell Physiol
June 2024
Department of Laboratory Animal Science, China Medical University, Shenyang, Liaoning, China.
Article Synopsis
- The study looked at how a substance called mogrol affects the treatment of a type of lung cancer called non-small cell lung cancer (NSCLC) when combined with radiation therapy.
- Researchers found that mogrol helps make cancer cells more sensitive to radiation, which means the radiation can kill more cancer cells.
- They discovered that mogrol works by reducing the levels of a protein called USP22, which is linked to cancer growth, by using specific pathways in the cells.
Role of Radiation-related Lung Function Genes in Prognosis and Immune Infiltration of Lung Adenocarcinoma.
Comb Chem High Throughput Screen
April 2024
Department of Radiotherapy, Affiliated Hospital of Hebei University, Baoding, China.
Background: Lung adenocarcinoma (LUAD) is a common malignant tumor with no obvious clinical symptoms in its early stages. Patients can be divided into radiotherapysensitive groups (RS) and radiotherapy-resistant groups (RR) due to their varying conditions. The therapeutic effect of radiotherapy is quite different between the two groups.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!