Evaluation of treatments on physiological attributes and production of diterpenoids in (Burm.f.) Nees.

Background: An ancient text on plant life mentions the use of horticulture treatments, (KJ) and (PG) (cow milk, cow ghee, cow curd, cow dung, and cow urine) to enhance the efficiency of plants.

Aim: An experiment was conducted to evaluate the effect of KJ and PG application on total leaf area, leaf area index (LAI) leaf area duration (LAD), crop growth rate (CGR), relative growth rate (RGR), and net assimilation rate (NAR); production of andrographolide (A1), neoandrographolide (A2), and 14-deoxy-11,12-didehydroandrographolide (A3) of the (Burm.f.) Nees.

Materials And Methods: The experiment was conducted in randomized block design with six treatments, namely control, KJ, PG, farmyard manure, inorganic fertilizer, and humic acid. Simultaneous detection of contents was carried out using reversed-phase-ultra-flow liquid chromatography (RP-UFLC). The parameters were studied at 30, 60, 90, and 120 days after sowing (DAS).

Results: KJ produced higher leaf area and LAI at 90 DAS, LAD between 90 and 120 DAS. PG produced higher CGR, RGR, and NAR between 60 and 90 DAS. RP-UFLC analysis revealed the maximum amount of ingredients at 120 DAS. At this stage, PG treatment recorded the maximum amount of A1, A2, and A3.

Conclusion: and treatments were better or at par on physiological parameters and production of diterpenoids of .