Maternal transfer of florfenicol impacts development and disrupts metabolic pathways in F1 offspring zebrafish by destroying mitochondria.

Ecotoxicol Environ Saf

Institute of Quality Standards and Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture and Rural Affairs, Beijing 100081, China. Electronic address:

Published: March 2023

AI Article Synopsis

  • Maternal exposure to the antibiotic florfenicol in zebrafish led to significant adverse effects in their offspring, including reduced fecundity, hatchability, and body length, as well as various developmental malformations.
  • Metabolomic and transcriptomic analyses indicated metabolic disruptions, such as impaired amino acid and lipid metabolism, along with decreased ATP levels and oxidative stress, linked to mitochondrial dysfunction in the F1 generation.
  • The study emphasizes that even non-direct exposure to environmental antibiotics like florfenicol can have lasting toxic effects on future generations, highlighting the importance of understanding environmental health risks.

Article Abstract

Maternal exposure to antibiotics existing in the environment is a predisposing factor for developmental malformation with metabolic disorders in offspring. In this study, female zebrafish (3 months) were exposed to 0.05 mg/L and 0.5 mg/L florfenicol (FF) for 28 days. After pairing and spawning with healthy male fish, F1 embryos were collected and developed to 5 d post-fertilization (dpf) in clear water. And the adverse effects on the F1 generation were examined thoroughly. The fecundity of F0 female fish and the hatchability, mortality, and body length of F1 larvae significantly decreased in the treatment group. Meanwhile, multi-malformation types were found in the exposure group, including delayed yolk sac absorption, lack of swim bladder, and spinal curvature. Metabolomic and transcriptomic results revealed alterations in metabolism with dysregulation in tricarboxylase acid cycle, amino acid metabolism, and disordered lipid metabolism with elevated levels of glycerophospholipid and sphingolipid. Accompanying these metabolic derangements, decreased levels of ATP and disordered oxidative-redox state were observed. These results were consistent with the damaged mitochondrial membrane potential and respiratory chain function, suggesting that the developmental toxicity and perturbed metabolic signaling in the F1 generation were related to the mitochondrial injury after exposing F0 female zebrafish to FF. Our findings highlighted the potential toxicity of FF to offspring generations even though they were not directly exposed to environmental contaminants.

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Source
http://dx.doi.org/10.1016/j.ecoenv.2023.114597DOI Listing

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