is a traditional Chinese herb of medicinal importance, with indole terpene alkaloids as its main active components. To study the expression of the most suitable housekeeping reference genes in . , the root bark, stem segments, leaves and inflorescences of four different parts of . were used as materials in this study. The expression stability of 10 candidate housekeeping reference genes (, , , , , , -, , , and ) was assessed through real-time fluorescence quantitative PCR, GeNorm, NormFinder, BestKeeper, ΔCT, and RefFinder. The results showed that - was stably expressed in all four parts of . and was the most suitable housekeeping gene. Based on the coexpression pattern of genome, full-length transcriptome and metabolome, the key candidate targets of 18 related genes (, , , , , , , , , -, , -, -, -, , , , and ) involved in the alkaloid biosynthesis were obtained. The expression of 18 related enzyme genes were analyzed by qRT-PCR using the housekeeping gene - as a reference. The results showed that these genes' expression and gelsenicine content trends were correlated and were likely to be involved in the biosynthesis of the alkaloid, gelsenicine.
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