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Bovine besnoitiosis: Assessment of the diagnostic accuracy of three different tests using a Bayesian latent class model approach and clinical characterization of the disease.
Prev Vet Med
December 2024
Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia, BO 40064, Italy. Electronic address:
Bovine besnoitiosis, a disease caused by the tissue cyst-forming apicomplexan Besnoitia besnoiti, is re-emerging in Europe, leading to significant impairment of health and production, as well as economic losses. The early detection of the disease is of the utmost importance for the implementation of effective control measures, yet this is a challenge due to the lack of specific early clinical signs. The objectives of our study were 1) to estimate the diagnostic accuracy of three tests to detect B.
View Article and Find Full Text PDFComparative diagnostic performance of recombinant Tams1 protein based dot-ELISA in detecting tropical theileriosis in naturally infected cattle.
Sci Rep
January 2025
Department of Veterinary Parasitology, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001, India.
This study is the first to conduct a sero-surveillance of Bovine Tropical Theileriosis (BTT) caused by the protozoan parasite Theileria annulata (T. annulata) using a recombinant Tams1 protein-based dot-ELISA in cattle, and to compare its efficacy with plate-ELISA, single PCR, nested PCR, and blood microscopy. The goal was to identify the most effective method for the early and accurate detection of theileriosis, which significantly impacts livestock through reduced milk yield and increased mortality.
View Article and Find Full Text PDFIroning out differences in attenuation and blooming artifact in acute stroke thrombi.
Sci Rep
January 2025
Medical Physics, Clinic for Radiology, University of Münster and University Hospital of Münster, 48149, Münster, Albert-Schweitzer-Campus 1, Building A1, Germany.
This study aims to improve our understanding of acute ischemic stroke clot imaging by integrating CT attenuation information with MRI susceptibility signal of thrombi. For this proof-of-principle experimental study, fifty-seven clot analogs were produced using ovine venous blood with a broad histological spectrum. Each clot analog was analyzed to determine its RBC content and chemical composition, including water, Fe III, sodium, pH, and pO2.
View Article and Find Full Text PDFThe appropriate nutrient conditions for methicillin-resistant Staphylococcus aureus and Candida albicans dual-species biofilm formation in vitro.
Sci Rep
January 2025
Department of Biological and Medical Sciences, Charles University, Faculty of Pharmacy in Hradec Králové, 2089, Zborovská, Hradec Králové, 500 03, Czech Republic.
Polymicrobial biofilms, the reason for most chronic wound infections, play a significant role in increasing antibiotic resistance. The in vivo effectiveness of the new anti-biofilm therapy is conditioned by the profound evaluation using appropriate in vitro biofilm models. Since nutrient availability is crucial for in vitro biofilm formation, this study is focused on the impact of four selected cultivation media on the properties of methicillin-resistant Staphylococcus aureus and Candida albicans dual-species biofilms.
View Article and Find Full Text PDFAttomolar-sensitive milk fever sensor using 3D-printed multiplex sensing structures.
Nat Commun
January 2025
School of Animal Sciences, Virginia Tech, Blacksburg, USA.
The diagnosis of milk fever or hypocalcemia in lactating cows has a significant economic impact on the dairy industry. It is challenging to identify asymptomatic subclinical hypocalcemia (SCH) in transition dairy cows. Monitoring subclinical hypocalcemia in milk samples can expedite treatment and improve the health, productivity, and welfare of dairy cows.
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