Cloning, expression and purification of antigenic fragments of the protein and its value in serology.

Background And Objectives: The detection of is usually done through culture. With the change of the smallest effective factor in culture, we face the lack of growth of these bacteria, which is one of the important reasons to find a suitable alternative for the diagnosis of this bacterium. is a protected gene in this bacterium. The aim of this was to evaluate the ability of antigenic regions of protein to bind to patients' serum antibodies.

Materials And Methods: Antigenic regions of protein were predicted using IEDB software with five different methods: Emini Surface Accessibility Prediction, Kolaskar and Tongaonkar Antigenicity, Chou and Fasman beta turn prediction, Karplus and Schulz flexibility scale, Ellipro-Epitope prediction based upon structural protrusion. Antigenic regions of gene was clonned, expressed and purified. The antigenicity of this recombinant protein against the antibodies in the serum of people infected with infections was checked in western blotting.

Results: The results showed that the antigenic regions of the protein was producted and its antigenicity was demonstrated in western blotting. Moreover, all sera from patients infected with reacted to the recombinant antigen.

Conclusion: Specimens from people infected with infection was positive in Western blotting suggesting that protein has antigenic properties. Therefore, it can be used as a suitable candidate for the design of diagnostic kits and vaccine.