Prevalence of main quinolones and carbapenems resistance genes in clinical and veterinary strains.

Iran J Microbiol

Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

Published: December 2022

Background And Objectives: Antibiotics-resistant strains are considered one of the most important causes of human and animal infections worldwide. The aim of current study was to detect common resistance (carbapenems and quinolones) genes by PCR.

Materials And Methods: A total of 100 strains isolated from human urinary tract infection and 20 isolated strains of aborted sheep embryos were collected. PCR was performed using specific primers to detect the resistance genes.

Results: Overall, among the quinolones resistance genes, resistance gene had the highest frequency (48%) and among carbapenem resistance genes, resistance gene had the highest frequency (45%). The frequency of resistance genes, IMP (28.45%), KPC (9.5%), VIM (9.15%), NDM (7.20%) were observed in clinical and veterinary strains, respectively. According to the results, 38.6% of strains had at least one from five genes of resistance to quinolones. The lowest frequency of resistance gene was related to , which was observed in only 29 (24.2%) strains.

Conclusion: Monitoring of carbapenem and quinolone resistance in pathogenic to humans and animals has an important value in revising treatment guidelines and the national public health, and plays an important role in preventing the spread of resistant strains.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867622PMC
http://dx.doi.org/10.18502/ijm.v14i6.11259DOI Listing

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