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SubtiToolKit: a bioengineering kit for Bacillus subtilis and Gram-positive bacteria.
Trends Biotechnol
March 2025
Department of Bioengineering, Imperial College London, London, UK; Imperial College Centre for Synthetic Biology, Imperial College London, London, UK.
Building DNA constructs of increasing complexity is key to synthetic biology. Golden Gate (GG) methods led to the creation of cloning toolkits - collections of modular standardized DNA parts hosted on hierarchic plasmids, developed for yeast, plants, Gram-negative bacteria, and human cells. However, Gram-positive bacteria have been neglected.
View Article and Find Full Text PDFA new removable connecting device for MARPE expander with non-common path of insertion.
Zhong Nan Da Xue Xue Bao Yi Xue Ban
October 2024
Xiangya Stomatological Hospital and Xiangya School of Stomatology, Central South University; Hunan Engineering Research Center for Digital Intelligence and Personalized Medicine; Hunan 3D Printing Engineering Research Center of Oral Care, Changsha 410008.
Objectives: Maxillary transverse deficiency is a common malocclusion frequently observed in orthodontic clinics. Miniscrew-assisted rapid palatal expansion (MARPE) not only produces greater skeletal expansion but also offers advantages such as simple miniscrew implantation without flap elevation, enhanced patient comfort, and an expanded age range and indications for palatal expansion. However, the fixed connection between the expander and the miniscrews makes the expander difficult to remove, significantly hindering its clinical application.
View Article and Find Full Text PDFAltered Bile Acids and Pouch Microbiota Composition in Patients With Chronic Pouchitis.
Inflamm Bowel Dis
March 2025
Division of Gastroenterology & Hepatology, Mayo Clinic, Rochester, MN, USA.
UnifiedGreatMod: A New Holistic Modelling Paradigm for Studying Biological Systems on a Complete and Harmonious Scale.
Bioinformatics
March 2025
Department of Computer Science, University of Turin, Torino, 10123, Italy.
Motivation: Computational models are crucial for addressing critical questions about systems evolution and deciphering system connections. The pivotal feature of making this concept recognisable from the biological and clinical community is the possibility of quickly inspecting the whole system, bearing in mind the different granularity levels of its components. This holistic view of system behaviour expands the evolution study by identifying the heterogeneous behaviours applicable, for example, to the cancer evolution study.
View Article and Find Full Text PDFSupporting islet function in a PVDF membrane based macroencapsulation delivery device by solvent non-solvent casting using PVP.
PLoS One
March 2025
Cell Biology-Inspired Tissue Engineering (cBITE), MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, The Netherlands.
Type 1 diabetic (T1D) patients are life-long dependent on insulin therapy to keep their blood glucose levels under control. An alternative cell-based therapy for exogenous insulin injections is clinical islet transplantation (CIT). Currently the widespread application of CIT is limited, due to risks associated with the life-long use of immunosuppressive drugs to prevent rejection of donor cells.
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