The transport of the CagA effector into gastric epithelial cells by the Cag Type IV secretion system (Cag T4SS) of Helicobacter pylori (H. pylori) is critical for pathogenesis. CagA is recruited to Cag T4SS by the Cagβ ATPase. CagZ, a unique protein in H. pylori, regulates Cagβ-mediated CagA transport, but the underlying mechanisms remain unclear. Here we report the crystal structure of the cytosolic region of Cagβ, showing a typical ring-like hexameric assembly. The central channel of the ring is narrow, suggesting that CagA must unfold for transport through the channel. Our structure of CagZ in complex with the all-alpha domain (AAD) of Cagβ shows that CagZ adopts an overall U-shape and tightly embraces Cagβ. This binding mode of CagZ is incompatible with the formation of the Cagβ hexamer essential for the ATPase activity. CagZ therefore inhibits Cagβ by trapping it in the monomeric state. Based on these findings, we propose a refined model for the transport of CagA by Cagβ.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9886983 | PMC |
| http://dx.doi.org/10.1038/s41467-023-36218-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Enhanced activity of autoimmune gastritis following Helicobacter pylori eradication therapy.
Clin J Gastroenterol
January 2025
Department of General Internal Medicine 2, Kawasaki Medical School General Medical Center, Okayama, Japan.
The relationship between autoimmune gastritis (AIG) and Helicobacter pylori (H. pylori) gastritis remains unclear, particularly whether there is any interaction. Herein, we report a case of early-stage AIG diagnosed in an elderly patient with highly active H.
View Article and Find Full Text PDFLow-level primary clarithromycin resistance of Helicobacter pylori in Reunion Island.
Infect Dis Now
January 2025
Introduction: The increasing resistance of Helicobacter pylori to clarithromycin leads to an ongoing adaptation of empirical first-line treatment for H. pylori infections.
Patients And Methods: Prospective study (2022-2023) of 364 patients with no previous treatment for H.
Complications of Post-Roux-en-Y Gastric Bypass: A Case of Excluded Stomach Perforation.
Cureus
December 2024
Trauma and Acute Care Surgery, Northeast Georgia Medical Center Gainesville, Gainesville, USA.
Roux-en-Y gastric bypass (RYGB) is a common surgical treatment for morbid obesity, but rare complications involving the excluded gastric remnant can pose significant challenges. A 65-year-old female with a history of RYGB presented with sudden onset of left upper quadrant abdominal pain, bloating, nausea, and loss of appetite. Laboratory tests revealed leukocytosis.
View Article and Find Full Text PDFgastritis in Jordanian children: persistence versus resolution.
Prz Gastroenterol
November 2023
Pediatric Department, King Abdullah University Hospital, Irbid, Jordan.
Introduction: () is the most common cause of infectious gastritis. is an infection that is typically acquired during childhood.
Aim: This study aims to describe children with infection and compare the clinicopathological features of children with resolved and persistent infection.
Effect of daphnetin, the coumarin derivative isolated from Daphne genus, on Helicobacter pylori adhesion to gastric epithelial cells.
Phytomedicine
December 2024
Beijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100073, China. Electronic address:
Background: Adherence of Helicobacter pylori to the surface of the gastric mucosa is the initial and crucial step for its survival and colonization in the harsh conditions of the stomach. We had previously demonstrated that daphnetin has anti-adhesion effect.
Purpose: This study aims to explore the mechanisms of daphnetin to reduce H.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!