Antihelminthic Activity of on Using an Automated High-Throughput Method.

Background: There are about 13 parasitic infections that are responsible for significant morbidity and mortality but have not received the attention they deserve; thus, they are now known as "neglected tropical diseases" (NTDs). This study was aimed at evaluating the antihelminthic activities of using an automated high-throughput method.

Methods: The antihelminthic activity effect of the extracts against . was determined using an automated high-throughput method. For the egg-hatching test, 100 L of embryonated egg suspension (60 eggs) was added to 100 L of various concentrations of extracts, levamisole, and 1.5% DMSO in a 96-well round-bottom microtitre plate. After mixing, the 96-well microplate was placed in WMicroTracker and incubated for 24 h at 25°C; the movements were recorded every 30 minutes. The same procedure was used for the larval motility assays, where 100 L of L1 or L2 larvae (50 larvae) were put in contact with 100 L of various concentrations of extracts.

Results: The ovicidal activity (hatching) had an IC of 1.4 mg/mL for the ethanol extract. The aqueous and ethanol extracts of . showed larvicidal activity on the L1 larvae with IC of 1.85 mg/mL and 2.4 mg/mL, respectively, as well as on the L2 larvae with IC values of 1.08 mg/mL and 1.02 mg/mL for the aqueous and ethanol extracts, respectively. These results showed that the aqueous extract exhibited a stronger inhibitory power on the hatching rate of parasites than ethanol extracts, while the contrary effect was observed for the larval motility assays.

Conclusion: This study provides scientific data on the use of . by the local population for the treatment of helminthiases. However, and toxicity tests are necessary to assess its activity and safety.