AI Article Synopsis
- The study focuses on multiplexed immunofluorescence imaging of formalin-fixed, paraffin-embedded (FFPE) specimens to identify various cell types while maintaining their spatial arrangement.
- This technique has been validated specifically for mouse tissues and helps in understanding the immune environment within cancer tissues.
- The method enables detailed analysis of both cancer morphology and cell types in FFPE sections, which could inform cancer development, treatment responses, and resistance.
Article Abstract
Multiplexed immunofluorescence imaging of formalin-fixed, paraffin-embedded (FFPE) specimens mounted on glass slides allow the identification of multiple cell phenotypes while retaining spatial and morphological context. Multiplex immunofluorescence protocols have already been developed and validated for mouse tissues. Immunophenotyping analysis reliably depicts the immune landscape of cancer tissues that has been demonstrated to influence cancer development and progression as well as to have an impact on therapy responsiveness and resistance. Here, we describe a method for multiplexed fluorescence image analysis, enabling analysis of mouse cancer morphology and cell phenotypes in FFPE sections.
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| http://dx.doi.org/10.1016/bs.mcb.2022.07.003 | DOI Listing |
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