Metabolomic and Transcriptome Analysis of the Inhibitory Effects of Strain Z-14 against Causing Vascular Wilt Diseases in Cucumber.

Controlling cucumber Fusarium wilt caused by f. sp. () with strains is a hot research topic. However, the molecular mechanism of underlying the biocontrol of cucumber wilt is rarely reported. In this study, strain Z-14 showed significant antagonistic activity against , and the control effect reached 88.46% via pot experiment. Microscopic observations showed that strain Z-14 induced the expansion and breakage of hyphae. The cell wall thickness was uneven, and the organelle structure was degraded. The combined analysis of metabolome and transcriptome showed that strain Z-14 inhibited the infection by inhibiting the synthesis of cell wall and cell membrane, energy metabolism, and amino acid synthesis of mycelium, inhibiting the clearance of reactive oxygen species (ROS) and the secretion of cell wall-degrading enzymes (CWDEs), thereby affecting mitogen-activated protein kinase (MAPK) signal transduction and inhibiting the transport function.