AI Article Synopsis
- * Researchers developed a method to incorporate human immune cells into HIOs by transplanting them into mice with a humanized immune system, enabling the study of immune interactions.
- * The study found that human immune cells migrate to the HIOs and respond to microbial exposure, activating the immune response and providing a new model for researching intestinal diseases.
Article Abstract
Human intestinal organoids (HIOs) derived from pluripotent stem cells provide a valuable model for investigating human intestinal organogenesis and physiology, but they lack the immune components required to fully recapitulate the complexity of human intestinal biology and diseases. To address this issue and to begin to decipher human intestinal-immune crosstalk during development, we generated HIOs containing immune cells by transplanting HIOs under the kidney capsule of mice with a humanized immune system. We found that human immune cells temporally migrate to the mucosa and form cellular aggregates that resemble human intestinal lymphoid follicles. Moreover, after microbial exposure, epithelial microfold cells are increased in number, leading to immune cell activation determined by the secretion of IgA antibodies in the HIO lumen. This in vivo HIO system with human immune cells provides a framework for future studies on infection- or allergen-driven intestinal diseases.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10264243 | PMC |
| http://dx.doi.org/10.1038/s41587-022-01558-x | DOI Listing |
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