One of the key metrics in the design of biosensors is the presence of an effective capture layer. Surface-immobilized proteins (especially as a part of antibody-antigen combinations) are the most commonly used capture ligands in biosensors. The surface coverage of these proteins in flow-based biosensors are affected by both the linker chemistry used to attach them as well as the microchannel geometry. We used streptavidin as a model protein to compare glutaraldehyde, EDC-NHS, sulfo-SMCC and sulfo-NHS-biotin as linkers inside straight, serpentine and square-wave microchannel geometries. We found that straight microchannels achieve the highest degree of protein immobilization compared to serpentine and square-wave microchannels, irrespective of the linker chemistry used. We also showed that for a given microchannel geometry, sulfo-NHS-biotin leads to the highest immobilization of streptavidin among all the linkers.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jbiotec.2023.01.005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characterization of the Dynamic Flow Response in Microfluidic Devices.
Small Methods
November 2024
Department of Mechanical Engineering, American University of Sharjah, Sharjah, UAE.
The purpose of this study is to characterize the dynamic response of fluid flow in microchannels, which can show significant delay times before reaching steady flow conditions. Two main sources of these delays are numerically and experimentally investigated, the hydraulic compliance which originates from the flexibility of the system components (microchannel, tubing, syringe, etc.), and the compressibility of the liquid dead volume in the setup, also known as the "bottleneck effect".
View Article and Find Full Text PDFMechanical confinement matters: Unveiling the effect of two-photon polymerized 2.5D and 3D microarchitectures on neuronal YAP expression and neurite outgrowth.
Mater Today Bio
December 2024
Department of Precision and Microsystems Engineering, Faculty of Mechanical Engineering, Delft University of Technology, Mekelweg 2, 2628 CD Delft, the Netherlands.
The effect of mechanical cues on cellular behaviour has been reported in multiple studies so far, and a specific aspect of interest is the role of mechanotransductive proteins in neuronal development. Among these, yes-associated protein (YAP) is responsible for multiple functions in neuronal development such as neuronal progenitor cells migration and differentiation while myocardin-related transcription factor A (MRTFA) facilitates neurite outgrowth and axonal pathfinding. Both proteins have indirectly intertwined fates via their signalling pathways.
View Article and Find Full Text PDFCFD Two-Phase Blood Model Predicting the Hematocrit Heterogeneity Inside Fiber Bundles of Blood Oxygenators.
Ann Biomed Eng
November 2024
LaBS - Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133, Milano, Italy.
Article Synopsis
- The study highlights the limitations of treating blood as a single-phase fluid in simulations, noting that hematocrit heterogeneity is present during blood flow in fiber bundles of oxygenators.
- A two-phase blood model based on the Eulerian-Eulerian approach was developed to accurately predict RBC distribution and phase separation, utilizing data from previous microfluidic experiments.
- The model successfully demonstrated the ability to analyze hematocrit patterns linked to fiber bundle structures, providing insights for optimizing gas transfer in blood oxygenation processes.
Ultra-Stretchable Microfluidic Devices for Optimizing Particle Manipulation in Viscoelastic Fluids.
ACS Appl Mater Interfaces
November 2024
School of Mechanical and Mining Engineering, University of Queensland, St. Lucia, Brisbane, Queensland 4067, Australia.
Viscoelastic microfluidics leverages the unique properties of non-Newtonian fluids to manipulate and separate micro- or submicron particles. Channel geometry and dimension are crucial for device performance. Traditional rigid microfluidic devices require numerous iterations of fabrication and testing to optimize these parameters, which is time-consuming and costly.
View Article and Find Full Text PDFElectrotaxis of Self-Propelling Artificial Swimmers in Microchannels.
Phys Rev Lett
October 2024
Max Planck Institute for Dynamics and Self-Organization, Am Faßberg 17, 37077 Göttingen, Germany.
Biological microswimmers alter their swimming trajectories to follow the direction of an applied electric field, exhibiting electrotaxis. We show that synthetic active droplet microswimmers also autonomously change swimming trajectories in microchannels, even undergoing "U-turns," in response to an electric field, mimicking electrotaxis. We exploit such electrotaxis, in the presence of an external flow, to robustly tune the swimming trajectory of active droplets between wall-adjacent, oscillatory, and channel centerline swimming.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!