In this experiment, the polysaccharide was extracted from Pueraria lobata (Willd.) Ohwi, and its structural characteristics and bioactivity were investigated. The results showed that Pueraria lobata polysaccharide (PLP) was composed of fucose, arabinose, galactose, glucose, xylose, mannose in a molar proportion of 0.09:1.25:2.19:95.74:0.43:0.30 with a number molar masses (Mn) weight of 14.463 kDa. Besides, FT-IR, Methylation, and NMR analysis revealed that PLP were mainly composed of the main chain →4)-α-Glcp (1→ and →4,6)-α-Glcp (1→, and the branched chain α-Glcp (1→. In vitro experiment, the results showed that PLP could stimulate the expression of surface molecules on RAW264.7 and (T and B) lymphocytes proliferation, simultaneously to stimulate their cytokines secretion. In vivo experiment, the immune organ index, cytokine content, and T lymphocyte subtype in cyclophosphamide-induced immunosuppressed mice could be improved by PLP. These data proved that PLP could be used as a useful immunomodulator to enhance the immune activity of RAW264.7, T, and B cells and improve the immune function of cyclophosphamide-treated mice.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijbiomac.2023.123386 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative analysis of the complete chloroplast genome of Pueraria provides insights for species identification, phylogenetic relationships, and taxonomy.
BMC Plant Biol
December 2024
College of Pharmacy, Dali University, Dali, 671000, China.
Background: Pueraria is an edible and medicinal raw material, which is of great value to the pharmaceutical and food industries. Nonetheless, due to morphological diversity and complex domestication history, the classification of Pueraria plants is ambiguous. As the varieties on the market are mixed, the species are difficult to distinguish, and their morphological characteristics are similar to the physical and chemical properties.
View Article and Find Full Text PDF[Fermentation technology in application of Puerariae Lobatae Radix with medicinal and edible properties: a review].
Zhongguo Zhong Yao Za Zhi
November 2024
Key Laboratory of Modern Chinese Medicine Formulations of Ministry of Education, Jiangxi University of Chinese Medicine Nanchang 330004, China.
Puerariae Lobatae Radix, as a traditional Chinese medicine(TCM) with both medicinal and edible properties, possesses effects such as relieving muscle tension and fever, generating fluids and quenching thirst, and unblocking the meridians and collaterals. Modern fermentation technology, combined with microecology and modern bioengineering, can regulate the fermentation process and efficiently produce fermentation products. In recent years, modern fermentation technology has been widely applied in TCM, enhancing or altering efficacy, reducing toxicity, and expanding the scope of clinical applications.
View Article and Find Full Text PDFIntegrative Analyses of Metabolome and Transcriptome Reveal Regulatory Network of Puerarin Biosynthesis in var. .
Molecules
November 2024
School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
Kudzu, scientifically known as var. (Willd.) Maesen & S.
View Article and Find Full Text PDFAn ultrasonic degraded polysaccharide extracted from Pueraria lobata ameliorate ischemic brain injury in mice by regulating the gut microbiota and LPS-TLR4 pathway.
Ultrason Sonochem
December 2024
Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. Electronic address:
Ischemia brain injury is closely associated with the gut microbiota. Polysaccharides, as a typical prebiotic, have been extensively employed in stroke treatment. In our previous study, Pueraria lobata polysaccharide (PLP-3) with antioxidant activity was prepared via water extraction and alcohol precipitation combined with ultrasonic degradation.
View Article and Find Full Text PDFAnti-rheumatic arthritis efficacy of Pueraria montana extract against type-II collagen-induced rheumatoid arthritis rat model an in vitro and in vivo assessment.
J Ethnopharmacol
November 2024
The Rheumatology and Immunology Department, The Affiliated Hospital of Jiangnan University, Wuxi, 214000, China. Electronic address:
Ethnopharmacological Relevance: Pueraria montana (PM) is a Chinese medicinal herb used to treat alcoholism, inflammation, swelling, and anti-apoptosis. However, the mechanisms and active compounds of PM remain poorly understood.
Aim Of The Study: Chronic inflammatory diseases such as rheumatoid arthritis (RA) can affect multiple joints.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!