INVESTIGATION OF ALCOHOL DEHYDROGENASE (ADH3) GENE POLYMOPHISM IN PATIENTS WITH CHRONIC ALCOHOLIC PANCRATITIS IN AZERBAIJAN POPULATION.

The aim of the study is to determine the single nucleotide polymorphism of the ADH3 gene, which is involved in the development of chronic alcoholic pancreatitis in the Azerbaijani population. Seventy patients (51 with chronic alcoholic pancreatitis, 19 with chronic non-alcoholic pancreatitis) and 90 healthy individuals (55 smokers and 35 non- drinkers) were included in the study. Genomic DNA was isolated from venous blood based on the kit protocol. Genotypes were determined on agarose gel using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) methods. To study the specificity of the ADH3 gene polymorphism in alcohol-dependent people, genotypes and alleles were compared in patients with chronic alcoholic pancreatitis (CAP) and "practically healthy " alcohol abusers. It was found that in both groups, the ADH3*1/ADH3*2 genotype predominates. It was also found that in the Azerbaijani population the frequency of ADH3*1 and ADH3*2 alleles is equally divided. From the data obtained, it can be clearly stated that the correlation of the ADH3*1 allele of the ADH3 gene polymorphism with an increased risk of the disease is not detected (p=0.876). When comparing genotypes and alleles in patients with chronic pancreatitis of non-alcoholic origin and non-drinkers, the predominance of the heterozygous ADH3*1 gene in both groups was also revealed without statistical significance (p=0.777). In the Azerbaijani population, the association between the polymorphism of the ADH3 gene genotypes and the development of CAP was not revealed. The predominance of the ADH3*1/ADH3*2 genotype explains the low incidence of both CAP and alcohol-dependent people in the Azerbaijani population.