AI Article Synopsis
- - Cholangiocarcinoma (CCA) is a serious cancer often diagnosed late, with no reliable early detection methods available yet.
- - Researchers have developed a new technology called FLEX that uses nanoplasmonic sensing to analyze tumor-derived extracellular vesicles (tEVs) from liquid biopsies, enabling more sensitive and robust detection of cancer biomarkers.
- - The FLEX assay not only identified key tumor markers in CCA but also showed better diagnostic capabilities than existing methods, achieving a high accuracy in detecting CCA from clinical bile samples.
Article Abstract
Cholangiocarcinoma (CCA) is a fatal disease often detected late in unresectable stages. Currently, there are no effective diagnostic methods or biomarkers to detect CCA early with high confidence. Analysis of tumor-derived extracellular vesicles (tEVs) harvested from liquid biopsies can provide a new opportunity to achieve this goal. Here, an advanced nanoplasmonic sensing technology is reported, termed FLEX (fluorescence-amplified extracellular vesicle sensing technology), for sensitive and robust single EV analysis. In the FLEX assay, EVs are captured on a plasmonic gold nanowell surface and immunolabeled for cancer-associated biomarkers to identify tEVs. The underlying plasmonic gold nanowell structures then amplify EVs' fluorescence signals, an effective amplification process at the single EV level. The FLEX EV analysis revealed a wide heterogeneity of tEVs and their marker levels. FLEX also detected small tEVs not detected by conventional EV fluorescence imaging due to weak signals. Tumor markers (MUC1, EGFR, and EPCAM) are identified in CCA, and this marker combination is applied to detect tEVs in clinical bile samples. The FLEX assay detected CCA with an area under the curve of 0.93, significantly better than current clinical markers. The sensitive and accurate nanoplasmonic EV sensing technology can aid in early CCA diagnosis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10015870 | PMC |
| http://dx.doi.org/10.1002/advs.202205148 | DOI Listing |
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