Actinobacteria are a rich source of bioactive molecules, and genome sequencing has shown that the vast majority of their biosynthetic potential has yet to be explored. However, many of their biosynthetic gene clusters (BGCs) are poorly expressed in the laboratory, which prevents discovery of their cognate natural products. To exploit their full biosynthetic potential, better understanding of the signals that promote the expression of BGCs is needed. Here, we show that the human stress hormone epinephrine (adrenaline) elicits siderophore production by Actinobacteria. Catechol was established as the likely eliciting moiety, since similar responses were seen for catechol and for the catechol-containing molecules dopamine and catechin but not for related molecules. Exploration of the catechol-responsive strain Streptomyces sp. MBT84 using mass spectral networking revealed elicitation of a BGC that produces the angucycline glycosides aquayamycin, urdamycinone B and galtamycin C. Heterologous expression of the catechol-cleaving enzymes catechol 1,2-dioxygenase or catechol 2,3-dioxygenase counteracted the eliciting effect of catechol. Thus, our work identifies the ubiquitous catechol moiety as a novel elicitor of the expression of BGCs for specialized metabolites.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9814775PMC
http://dx.doi.org/10.1038/s42004-022-00632-4DOI Listing

Publication Analysis

Top Keywords

ubiquitous catechol
8
catechol moiety
8
elicits siderophore
8
biosynthetic potential
8
expression bgcs
8
catechol
6
moiety elicits
4
siderophore angucycline
4
angucycline production
4
production streptomyces
4

Similar Publications

The L-type Ca channel (Ca1.2) is essential for cardiac excitation-contraction coupling. To contribute to the inward Ca flux that drives Ca-induced-Ca-release, Ca1.

View Article and Find Full Text PDF

Free radicals are ubiquitous in biological systems, being responsible for pathogenesis of degenerative diseases and participating in vitally important biochemical processes, which are mediated by radical regulatory agents. The effects of the aliphatic amine substituents in the catechol-derived Mannich bases on their antioxidant and pro-oxidant activity were investigated. It has been found that the presence of catechol moiety in the structure of Mannich bases allows them to act as Cu(II) reductants, efficient Fe(II) chelators and potent DPPH radical scavengers.

View Article and Find Full Text PDF

Investigating the gas-phase reaction mechanism of catechol with ozone: Product analysis and insights.

Environ Pollut

October 2023

Radiation, Chemicals and Environmental Hazards, Toxicology Department, UK Health Security Agency, Didcot, OX11 0RQ, United Kingdom.

Volatile aromatic compounds (VOCs) are ubiquitous in the environment, they can be emitted from biogenic and anthropogenic sources. They can contribute to the formation of many products leading to the formation of secondary organic aerosols (SOA). The products of the gas phase reaction of 1,2-benzenediol (catechol) with ozone were studied in a simulation chamber at atmospheric pressure and 294 ± 2 K in presence of different levels of relative humidity (0-60%).

View Article and Find Full Text PDF

The key to 2,6-dichloro-1,4-benzoquinone reproductive toxicity and green tea detoxification: Covalent binding and competitive binding.

Ecotoxicol Environ Saf

November 2024

State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, 999078, Macao, China. Electronic address:

Halobenzoquinones (HBQs) are ubiquitous disinfection by-products (DBPs) in chlorinated drinking water with various health risks including reproductive toxicity, while the potential mechanisms are still unclear. Although green tea exhibits common detoxifying properties, its ability to mitigate the toxicity of HBQs still needs to be further deepened and explored. This study attempted to investigate the possible mechanism of the most common HBQ, 2,6-dichloro-1,4-benzoquinone (2,6-DCBQ) induced reproductive toxicity and elucidate the protective effect of green tea using a series of liquid chromatography-tandem mass spectrometry (LC-MS) approaches.

View Article and Find Full Text PDF

Effects of dietary curcumin on gene expression: An analysis of transcriptomic data in mice.

Pathol Res Pract

November 2024

Center for Global health Research, Saveetha Medical College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India; Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:

Background: Curcumin, a ubiquitous polyphenol in turmeric, possesses many anti-cancer and anti-inflammatory properties. These therapeutic effects are largely resultant of curcumin's ability to modulate global gene expression. Bioinformatics-based approaches for analyzing differential gene expression are effective tools in gaining a more profound understanding of the underlying mechanisms of action.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!