Objective: Diabetic nephropathy (DN) is the leading cause of end-stage renal disease around the world. This study investigated the role of microRNA (miR)-29b-3p in DN and the mechanism of the miR-29b-3p/EZH2 axis in DN.
Methods: Peripheral blood samples of DN patients were collected and miR-29b-3p and EZH2 expression levels were evaluated using RT-qPCR. DN mouse models were successfully established, and then treated with miR-29b-3p overexpression or EZH2 silence. IL-1β, IL-6, and TNF-α levels were assessed by ELISA. Blood glucose, serum creatinine (Scr), 24-h urine volume, 24-h urine protein, and blood urea nitrogen (BUN) levels were examined by automatic biochemical analyzer detection. HE staining was performed to observe the renal histopathology, and TUNEL staining was implemented to test apoptosis in renal tissues. The binding relationship between miR-29b-3p and EZH2 was validated by using a bioinformatics website and dual luciferase reporter gene assay.
Results: miR-29b-3p was lowly expressed, and EZH2 was highly expressed in patients with DN. Overexpressing miR-29b-3p or silencing EZH2 attenuated renal dysfunction, suppressed inflammation and apoptosis, and relieved renal injuries in mice with DN. miR-29b-3p inhibited EZH2, and miR-29b-3p overexpression mitigated renal injuries in DN mice by repressing EZH2.
Conclusion: miR-29b-3p suppresses EZH2 expression thereby inhibiting the progression of DN in mice.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s42000-022-00426-2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Prognostic Significance of miRNA Subtypes in Melanoma: A Survival Analysis and Correlation with Treatment Response Across Patient Stages.
Biomedicines
December 2024
Discipline of Dermatology, "Victor Babes" University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania.
Background And Objectives: Melanoma remains a leading cause of skin cancer mortality despite advancements in targeted therapies and immunotherapies. MicroRNAs (miRNAs) have emerged as potential biomarkers for cancer prognosis and treatment response. This study aims to analyze survival outcomes according to various miRNA subtypes, assess the association between specific miRNAs and treatment response, and include patient staging to evaluate their prognostic significance.
View Article and Find Full Text PDFIntegrative Multi-Omics Approach in Vascular Ehlers-Danlos Syndrome: Further Insights into the Disease Mechanisms by Proteomic Analysis of Patient Dermal Fibroblasts.
Biomedicines
November 2024
Division of Biology and Genetics, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy.
: Dominant mutations in are known to cause vascular Ehlers-Danlos syndrome (vEDS) by impairing extracellular matrix (ECM) homeostasis. This disruption leads to the fragility of soft connective tissues and a significantly increased risk of life-threatening arterial and organ ruptures. Currently, treatments for vEDS are primarily symptomatic, largely due to a limited understanding of its underlying pathobiology and molecular mechanisms.
View Article and Find Full Text PDFAnalysing the relevance of TGF-β and its regulators in cervical cancer to identify therapeutic and diagnostic markers.
Gene
February 2025
Department of Biochemistry, Karpagam Academy of Higher Education, Coimbatore 641021, Tamil Nadu, India; Centre for Cancer Research, Karpagam Academy of Higher Education, Coimbatore 641021, Tamil Nadu, India. Electronic address:
The role of transforming growth factor-beta (TGF-β) is dual, such that, it inhibits tumor development in initial stage and promotes metastasis in later stage. The present study is aimed to analyse the relevance of different types of TGF-β and their receptors on the overall survival (OS) and TGF-β driven gene expression in individuals with cervical cancer (CC) using ONCODB and GEPIA databases. The in-silico gene expression analysis showed, TGF-β1 and TGFβR2 are upregulated in cells infected with human papilloma virus (HPV)16, whereas, TGF-β2, TGFβR1 and TGFβR3 expression were downregulated.
View Article and Find Full Text PDFCapecitabine regulates proliferation and apoptosis of ovarian cancer SKOV3 cells via the miR-29b-3p/MMP16 molecular axis.
Am J Transl Res
November 2024
Department of Women's Health, Huzhou Maternity and Child Health Care Hospital Huzhou 313000, Zhejiang, China.
Objective: To investigate the molecular mechanism by which capecitabine regulates the proliferation and apoptosis of ovarian cancer SKOV3 cells through the miR-29b-3p/MMP16 axis.
Methods: SKOV3 ovarian cancer cells were treated with capecitabine, miR-29b-3p mimics, miR-29b-3p inhibitor, and MMP16 siRNA. Cell proliferation was measured using the CCK-8 assay, and apoptosis was assessed by flow cytometry.
Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Loaded Mir-29-3p Targets AhR to Improve Juvenile Idiopathic Arthritis via Inhibiting the Expression of IL-22 in CD4 T Cell.
Stem Cell Rev Rep
December 2024
Pediatric Immunology and Rheumatology Department, School of Medicine, Chief Physician, Chengdu Women's and Children's Central Hospital, University of Electronic Science and Technology of China, No.1617, Riyue Avenue, Qingyang District, Chengdu, Sichuan, China.
Article Synopsis
- Juvenile idiopathic arthritis (JIA) is a common chronic inflammatory disease in children, and this study explores how human umbilical cord mesenchymal stem cell-derived exosomes (HUCMSCs-Exos) influence JIA by delivering miR-29-3p to inhibit IL-22 expression in T cells.
- In experiments with a collagen-induced arthritis mouse model and JIA patient T cells, results showed that HUCMSCs-Exos significantly reduced IL-22 levels while increasing miR-29-3p levels, reversing the expression of related genes in T cells.
- Ultimately, the study concludes that HUCMSCs-Exos targeting AhR through miR-29-3p may offer a
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!