Strain Subjected to Long-Term Exposure to svar. Larvicides Displays an Altered Transcriptional Response to Zika Virus Infection.

svar. (Bti) larvicides are effective in controlling ; however, the effects of long-term exposure need to be properly evaluated. We established an strain that has been treated with Bti for 30 generations (RecBti) and is still susceptible to Bti, but females exhibited increased susceptibility to Zika virus (ZIKV). This study compared the RecBti strain to a reference strain regarding: first, the relative transcription of selected immune genes in ZIKV-challenged females (F) with increased susceptibility detected in a previous study; then, the whole transcriptomic profile using unchallenged females (F). Among the genes compared by RT-qPCR in the ZIKV-infected and uninfected females from RecBti (F) and the reference strain, , , , , , and showed a trend of repression in RecBti infected females. The transcriptome of RecBti (F) unchallenged females, compared with a reference strain by RNA-seq, showed a similar profile and only 59 differentially expressed genes were found among 9202 genes analyzed. Our dataset showed that the long-term Bti exposure of the RecBti strain was associated with an alteration of the expression of genes potentially involved in the response to ZIKV infection in challenged females, which is an important feature found under this condition.