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Metabolic Engineering of for High-Level Production of ()-Acetoin from Low-Cost Raw Materials. | LitMetric

Metabolic Engineering of for High-Level Production of ()-Acetoin from Low-Cost Raw Materials.

Microorganisms

State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-Food Biorefinery, Guangxi Biomass Engineering Technology Research Center, Guangxi Academy of Sciences, 98 Daling Road, Nanning 530007, China.

Published: January 2023

AI Article Synopsis

  • - Acetoin is a valuable four-carbon compound used in various industries, particularly for creating optically pure forms that are crucial for pharmaceuticals and other applications.
  • - This study focused on enhancing the resistance of a specific strain to acetoin by overexpressing certain genes and optimizing key synthetic pathways, resulting in a high-producing strain.
  • - The engineered strain, GXASR-49RSF, successfully produced a significant amount of pure acetoin in a fermentation process using low-cost raw materials, demonstrating an effective and economical biotechnological method for production.

Article Abstract

Acetoin is an important four-carbon platform chemical with versatile applications. Optically pure ()-acetoin is more valuable than the racemate as it can be applied in the asymmetric synthesis of optically active α-hydroxy ketone derivatives, pharmaceuticals, and liquid crystal composites. As a cytotoxic solvent, acetoin at high concentrations severely limits culture performance and impedes the acetoin yield of cell factories. In this study, putative genes that may improve the resistance to acetoin for were screened. To obtain a high-producing strain, the identified acetoin-resistance gene was overexpressed, and the synthetic pathway of ()-acetoin was strengthened by optimizing the copy number of the key genes. The engineered . strain GXASR-49RSF produced 81.62 g/L ()-acetoin with an enantiomeric purity of 96.5% in the fed-batch fermentation using non-food raw materials in a 3-L fermenter. Combining the systematic approach developed in this study with the use of low-cost feedstock showed great potential for ()-acetoin production via this cost-effective biotechnological process.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867144PMC
http://dx.doi.org/10.3390/microorganisms11010203DOI Listing

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