Mammalian tuberculosis (TB) is a zoonotic disease mainly due to (). A current challenge for its eradication is understanding its transmission within multi-host systems. Improvements in long-read sequencing technologies have made it possible to obtain complete bacterial genomes that provide a comprehensive view of species-specific genomic features. In the context of TB, new genomic references based on complete genomes genetically close to field strains are also essential to perform precise field molecular epidemiological studies. A total of 10 strains representing each genetic lineage identified in France and in other countries were selected for performing complete assembly of their genomes. Pangenome analysis revealed a "closed" pangenome composed of 3900 core genes and only 96 accessory genes. Whole genomes-based alignment using progressive Mauve showed remarkable conservation of the genomic synteny except that the genomes have a variable number of copies of IS. Characteristic genomic traits of each lineage were identified through the discovery of specific indels. Altogether, these results provide new genetic features that improve the description of lineages. The availability of new complete representative genomes of will be useful to epidemiological studies and better understand the transmission of this clonal-evolving pathogen.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865570 | PMC |
http://dx.doi.org/10.3390/microorganisms11010177 | DOI Listing |
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