AI Article Synopsis

  • Lesion vitality demonstration in forensic pathology, especially in hanging cases, presents challenges that recent studies are addressing through immunohistochemistry.
  • The study focuses on evaluating FOXO3 protein expression in post-mortem cervical skin samples, using anti-FOXO3 antibodies, comparing cases of hanging to deaths from other causes.
  • Findings indicate that FOXO3 expression is significantly lower in hanging cases, suggesting its potential as a reliable marker for assessing the vitality of ligature marks.

Article Abstract

One of the most challenging issues in forensic pathology is lesion vitality demonstration, particularly in cases of hanging. Over the past few years, immunohistochemistry has been applied to this field with promising results. In particular, protein and transcription factors involved in the apoptotic process have been studied as vitality markers for the ligature mark. This study represents an implementation of our previous studies on ligature mark vitality demonstration. In this study, we evaluated the FOXO3 expression in post-mortem cervical skin samples through an immunohistochemical analysis. To evaluate FOXO3 expression, anti-FOXO3 antibodies (GTX100277) were used. The study group comprised 21 cases, 8 women and 13 men, whereas the control group consisted of 13 cases of subjects who died due to other causes. Decomposition and no clear circumstantial data were exclusion criteria. We found that FOXO3 is decreased in hanging cases compared with normal skin in other causes of death (p-value < 0.05). No differences were seen concerning the type of hanging material (hard or soft), type of hanging (complete or incomplete), and position of the knot. Our results suggest that FOXO3 depletion could be a valid immunohistochemical marker of ligature mark vitality.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866130PMC
http://dx.doi.org/10.3390/ijms24021396DOI Listing

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