A Potential Negative Regulatory Function of Myostatin in the Growth of the Pacific Abalone, .

Myostatin, also known as GDF8, is a member of the transforming growth factor-β (TGF-β) superfamily. In vertebrates, myostatin negatively regulates the growth of skeletal muscle. In invertebrates, it has been reported to be closely related to animal growth. However, knowledge concerning the molecular mechanisms involved in the myostatin regulation of molluscan growth is limited. In this study, we found that the hdh-myostatin open reading frame (ORF) comprised 1470 base pairs that encoded 489 amino acids and contained structural characteristics typical of the TGF-β superfamily, including a C-terminal signal peptide, a propeptide domain, and TGF-β region. Gene expression analysis revealed that mRNA was widely expressed at different levels in all of the examined tissues of . Nine single nucleotide polymorphisms (SNPs) were associated with the growth traits. RNA interference (RNAi) against mRNA significantly downregulated at days 1, 15, and 30 post injection, and the pattern was correlated with downregulation of the genes TGF-β receptor type-I (β I), activin receptor type-IIB ( IIB), and mothers against decapentaplegic 3 (3). After one month of the RNAi experiment, the shell lengths and total weights increased in the abalone, . The results of qRT-PCR showed that the mRNA level was higher in the slow-growing group than in the fast-growing group. These results suggest that hdh-myostatin is involved in the regulation of growth, and that these SNPs would be informative for further studies on selective breeding in abalone.