AI Article Synopsis
- * Results show that co-culture of SSC with SC leads to consistent expression of key markers and a stronger GC differentiation profile, while PB-MSC and SC show limited marker expression.
- * The findings suggest that SSC exhibit a more effective differentiation into germ cells compared to PB-MSC, highlighting the importance of stem cell type in GC derivation strategies.
Article Abstract
Although spermatogonial stem cells (SSC) constitute primary candidates for in vitro germ cell (GC) derivation, they are scarce and difficult to maintain in an undifferentiated state. Alternatively, mesenchymal stem cells (MSC) are also candidates for GC derivation due to their simplicity for culture and multipotential for transdifferentiation. The aim of the present study was to compare the GC differentiation potentials of bull peripheral blood-derived MSC (PB-MSC) and SSC using an in vitro 3D co-culture system with Sertoli cells (SC). Samples of PB-MSC or SSC co-cultures with SC were collected on days 0, 7, 14 and 21 and analyzed for pluripotency, GC and mesenchymal marker expression. Co-culture of PB-MSC+SC resulted in down-regulation of and up-regulation of at day 7. In comparison, co-culture of SSC+SC resulted in consistent expression of , and at day 14. During co-culture, SSC+SC increased the expression of , , and and activated the expression of , whereas co-culture of PB-MSC+SC only increased the expression of and . Thus, co-culture of bull PB-MSC+SC and SSC+SC in 3D SACS results in differential expression of pluripotency and GC markers, where bull SSC display a more robust GC differentiation profile compared to PB-MSC.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854759 | PMC |
| http://dx.doi.org/10.3390/ani13020318 | DOI Listing |
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