As a major mediator of cellular response to viral infection in mammals, Toll-like receptor 3 (TLR3) was proved to respond to double-stranded RNA (dsRNA). However, the molecular mechanism by which TLR3 functions in the viral infection response in teleosts remains to be investigated. In this study, the gene of the hybrid yellow catfish was identified and characterized by comparative genomics. Furthermore, multiple sequence alignment, genomic synteny and phylogenetic analysis suggested that the homologous TLR3 genes were unique to teleosts. Gene structure analysis showed that five exons and four introns were common components of TLR3s in the 12 examined species, and interestingly the third exon in teleosts was the same length of 194 bp. Genomic synteny analysis indicated that TLR3s were highly conserved in various teleosts, with similar organizations of gene arrangement. De novo predictions showed that TLR3s were horseshoe-shaped in multiple taxa except for avian (with a round-shaped structure). Phylogenetic topology showed that the evolution of TLR3 was consistent with the evolution of the studied species. Selection analysis showed that the evolution rates of TLR3 proteins were usually higher than those of TLR3-TIR domains, indicating that the latter were more conserved. Tissue distribution analysis showed that TLR3s were widely distributed in the 12 tested tissues, with the highest transcriptions in liver and intestine. In addition, the transcription levels of TLR3 were significantly increased in immune-related tissues after infection of exogenous and poly (I:C). Molecular docking showed that TLR3 in teleosts forms a complex with poly (I:C). In summary, our present results suggest that TLR3 is a pattern recognition receptor (PRR) gene in the immune response to pathogen infections in hybrid yellow catfish.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854889 | PMC |
http://dx.doi.org/10.3390/ani13020288 | DOI Listing |
Ecol Evol
January 2025
Unit of Evolutionary Biology/Systematic Zoology Institute for Biochemistry and Biology, University of Potsdam Potsdam Germany.
Genomics is an invaluable tool for conservation, particularly for endangered species impacted by wildlife trafficking. This study uses genomic data to provide new insights to aid conservation and management of endangered species, using as a case study the Yellow cardinal (), a bird endemic to southern South America severely affected by illegal trade and the transformation of its natural habitat. We explore population structure within the Yellow cardinal, delimiting management units and describing connectivity among them.
View Article and Find Full Text PDFMol Biol Rep
January 2025
Department of Agronomy and Plant Breeding Sciences, Agricultural College of Aburaihan, University of Tehran, Pakdasht, Iran.
Background: Tomato yellow leaf curl virus (TYLCV), tomato mosaic virus (ToMV), and Fusarium wilt are three of tomatoes' most important viral and fungal diseases.
Methods And Results: In this study, the application of molecular markers associated with tomato yellow leaf curl virus resistance gene (Ty1), tomato mosaic virus resistance gene (Tm2), and Fusarium wilt resistance gene (I-1) (linked marker) were evaluated. In order to optimize and use SNP markers (by HRM diagnostic method) and SCAR markers, segregating populations of tomatoes were produced by self-pollination of commercial hybrid cultivars.
Plant Dis
January 2025
Instituto Valenciano de Investigaciones Agrarias, Plant Protection and Biotechnology Center, Ctra Moncada-Naquera km 4.5, Moncada, Spain, 46113;
Peach-associated luteovirus (PaLV) belongs to the genus Luteovirus, family Tombusviridae. To date, PaLV has only been reported in peach (Prunus persica) and its presence detected in the Republic of Georgia (Wu et al., 2017), China (Zhou et al.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
January 2025
Universit Paris-Saclay, INRAE, UVSQ, VIM, Jouy-en-Josas, France.
Ten novel Gram-negative, aerobic, non-sporulating, yellow-pigmented rod-shaped bacterial strains motile by gliding were isolated from marine organisms/environments in French Polynesia. Three of them designated as 190524A05c, 190524A02b and 190130A14a were retrieved from orbicular batfish () mucus. Online database comparisons using 16S rRNA amplicons resulted in over 95% similarity to the genus .
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
School of Chemistry and Chemical Engineering Southeast University, Jiangning District, Nanjing, Jiangsu Province 211189, PR China. Electronic address:
Inkless paper made from photochromic materials has garnered significant interest owing to its potential to reduce both ink and paper pollution during production. In this research, we synthesized a dual-material film (EC-PVP/PGMEA/PMoA) and conducted a detailed investigation of its photochromic response to visible light and its microstructural properties. Initially, the film appeared as a translucent yellow, but upon exposure to visible light, it shifted to blue with a maximum absorption peak of 2.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!