1. Chicken liver nuclei were fractionated by disruption with ultrasound and subsequent precipitation with divatent cations. A small, protein-rich fraction (CS), representing less than 5% of the total nuclear DNA reacted strongly with antisera to dehistonized chicken liver chromatin. 2. Further fractionation of the CS by DNA-affinity chromatography yielded two DNA-binding immunoreactive proteins (approximate Mr 35 and 56 kD). 3. We have shown previously (Kiliańska et al., 1981, Kiliańska, 1984) that this DNA-binding component strongly inhibited the in vitro transcription of RNA.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0020-711x(87)90183-2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
BCAA interactions: how do they influence broiler performance, intestinal morphometry, lipid profile, and liver health?
Arch Anim Nutr
January 2025
Department of Animal Science, Federal University of Viçosa, Viçosa, MG, Brazil.
An experiment was conducted to assess the effects of the BCAA and their interactions on performance, carcass composition, lipid metabolism, liver health, and intestinal morphometry in broiler chickens. Male chickens ( = 1080) were randomly assigned into floor pens in a 3 × 3 factorial design with 3 dietary ratios of SID Leu:Lys (110, 150, and 190%), and 3 dietary ratios of SID Ile-Val:Lys (68-77, 78-87, and 88-97%). Performance parameters were assessed from 1 to 35 days of age.
View Article and Find Full Text PDFHealth risk assessment of ciprofloxacin, tetracycline, and oxytetracycline residues in raw, frozen, and boiled broiler chicken available in a local area of Bangladesh.
Front Antibiot
April 2024
Department of Food Technology and Nutritional Science, Mawlana Bhashani Science and Technology University, Tangail, Bangladesh.
Introduction: The misuse of antibiotics in poultry farming is a global issue.
Objective: The focus of this study was the health risk assessment of consumers from the determination of ciprofloxacin (CIP), tetracycline (TC), and oxytetracycline (OTC) in broiler chicken in the raw, frozen, and boiled stages using solid-phase extraction, high-performance liquid chromatography, and ultraviolet detection (SPE-HPLC-UV).
Materials And Methods: Chromatographic separation was achieved using 0.
Research on the metabolites and key metabolic enzymes of allocryptopine in chicken liver microsomes via stable isotope tracing technology.
J Pharm Biomed Anal
January 2025
Shanxi Key Lab. for modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China; College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China. Electronic address:
Allocryptopine (ALL), a principal active component of the novel veterinary medicine Bopu Powder®, has gained widespread application in the poultry farming sector for the effective management of Escherichia coli (E. coli) diarrhea. In order to explore the metabolites and the pivotal enzymes associated with ALL, this study was conducted employing an in vitro chicken liver microsomal incubation.
View Article and Find Full Text PDFExploring the metabolic patterns and response mechanisms of bile acids during fasting: A study with poultry as an example.
Poult Sci
December 2024
College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Zhengzhou 450046, China. Electronic address:
Fasting is beneficial to alleviate fatty liver, lose weight and improve reproductive function. However, previous studies have shown that, during fasting, disorders of bile acid metabolism were strongly associated with intestinal inflammation. The physiological and biochemical parameters and gene expression of multiple tissues of chickens at every critical time node were measured by ELISA and qPCR.
View Article and Find Full Text PDFRapid Determination of Organic and Inorganic Selenium in Poultry Tissues by Internal Extractive Electrospray Ionization Mass Spectrometry.
Anal Chem
January 2025
The Jiangxi Province Key Laboratory for Diagnosis, Treatment, and Rehabilitation of cancer in Chinese Medicine, Jiangxi University of Chinese Medicine, Nanchang 330004, P. R. China.
An online reactive internal extraction electrospray ionization (iEESI) method was developed for the rapid determination of organic and inorganic speciation information for selenium in poultry tissue samples without complex sample pretreatment. The addition of citric acid as a reducing agent to the internal extraction solvent of methanol/acetic acid (99:1, V/V) for iEESI resulted in the reduction of selenate in the sample to selenite, accompanied by the production of malic acid as an oxidation product. The quantitative analysis of selenate was conducted by using malic acid.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!