Mass Spectrometry Approaches for Detection and Determination of Prostaglandins from Biological Samples.

Methods Mol Biol

Bone-Muscle Research Center, College of Nursing & Health Innovation, University of Texas at Arlington, Arlington, TX, USA.

Published: January 2023

AI Article Synopsis

  • Accurate analysis of prostaglandins (PGs) from biological samples is essential to comprehend their roles in various bodily functions and diseases.* -
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is an effective method for detecting extremely low levels of PGs quickly and accurately from just one sample.* -
  • The text discusses LC-MS/MS techniques along with sample preparation methods, including off-line and on-line solid-phase extraction (SPE) for PG detection.*

Article Abstract

Accurate determination of prostaglandins (PGs) from biological samples is critical for understanding their biological functions and interactions during physiological and pathological processes. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a highly sensitive, accurate, and high-throughput approach for simultaneous detection of ultra-trace PGs from a single biological sample. Here we describe LC-MS/MS techniques and related sample pretreatment methods including both off-line and on-line SPE for the determination of PGs in biological samples.

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http://dx.doi.org/10.1007/978-1-0716-2966-6_25DOI Listing

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