AI Article Synopsis
- * The study presents a speedy method using HILIC-HRMS to effectively identify and quantify two types of plasmalogens: ethanolamine-plasmalogens and choline-plasmalogens in various biological samples.
- * It also includes a refined lipid extraction technique that works well across different biological contexts, enhancing the efficiency of the analysis.
Article Abstract
Analysis and quantification of ether-lipid phospholipid species-also known as plasmalogens-is a crucial step in the study of the biological functions played by these lipids. Application of analytical separation methods and high-resolution mass spectrometry has gained much attention in this regard, while resolution issues and time-consuming sequences interfered with these advances. Herein, we describe a simple and rapid method for the analysis of plasmalogen (Pl) species by HILIC-HRMS. This method is able to identify and quantify relative levels of ethanolamine-plasmalogens (PlsEtn) and choline-plasmalogens (PlsCho) in biological matrices such as whole blood, plasma, erythrocytes, and also retina. Moreover, we provide a detailed and modified lipid extraction method that is applicable to almost all biological matrices.
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| http://dx.doi.org/10.1007/978-1-0716-2966-6_22 | DOI Listing |
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