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Federal University of Sao Paulo (UNIFESP), Post-graduate Program in Sciences of Human Movement and Rehabilitation, Avenida Ana Costa 95, Santos- SP 11060-001, Brazil; Unievangelica, Post-graduate Program in Human Movement and Rehabilitation and in Pharmaceutical Sciences, Avenida Universitária Km 3,5, Anápolis, GP 75083-515, Brazil; Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology, Rua Pedro Ernesto 240, São José dos Campos, SP 12245-520, Brazil; Universidade Brasil, Post-graduate Program in Bioengineering and Biomedical Engineering, Rua Carolina Fonseca 235, São Paulo, SP 08230-030, Brazil. Electronic address:
Published: March 2023
AI Article Synopsis
Article Abstract
Leukemia is among the most common types of hematological cancers and the use of herbal medicines to prevent and treat leukemia are under quick development. Among several molecular pathways involved in leukemia pathogenesis and exacerbations, purinergic signaling is revealed as a key component. In the present study, the effects of two doses (5 ug/mL and 10 ug/mL) of Immunity-6™, a phytocomplex composed by beta-glucan, green tea (Camelia sinensis), chamomile (Matricaria chamomilla), and ascorbic acid (vitamin C) was tested in vitro, using chronic myelogenous leukemia cell line (K-562; 5 ×104/mL/well), which were challenged with lipopolysaccharide (LPS; 1 ug/mL) for 24 h. The results demonstrated that both doses of Immunity-6™ inhibited ATP release (p < 0.001) and P2×7 receptor at mRNA levels expression (p < 0.001). Purinergic inhibition by Immunity-6™ was followed by reduced release of proinflammatory cytokines IL-1beta (p < 0.001) and IL-6 (p < 0.001), while only 5 ug/mL of Immunity-6™ reduced the release of TNF-alpha (p < 0.001). Beyond to inhibit the release of pro-inflammatory cytokines, both doses of Immunity-6™ induced the release of anti-inflammatory cytokine IL-10 (p < 0.001), while only the higher dose (10 ug/mL) of Immunity-6™ induced the release of anti-inflammatory IL-1ra (p < 0.05) and klotho (p < 0.001). Thus, Immunity-6™ may be a promising adjuvant in the treatment of leukemia and further clinical trials are guaranteed.
