Selective fluorescence imaging of analytes is a challenge for monitoring diseases as homologues interfere with the imaging agents. Leucine aminopeptidase (LAP), a kind of protease, is related to tumor pathogenesis. The known LAP fluorescent probes based on leucine recognition have limited selectivity. Herein, a selective t-butyl-alanine recognition unit for LAP through the ligand regulation strategy is prepared as a new near-infrared (NIR) fluorescent probe () having a large Stokes shift of 214 nm and a high sensitivity with a detection limit of 168 mU/L. has an enhanced response toward LAP with NIR fluorescence at 656 nm based on intramolecular charge transfer. The probe is selective without being interfered with by biological enzymes including the aminopeptidase N (APN). can image LAP activity in living cells. It can also visualize the cell invasion and migration processes. is employed in the real-time imaging of LAP in tumor-bearing nude mice and guides in the accurate resection of breast tumors. It also distinguishes tumor tissues from normal with a high tumor-to-normal ratio (9.8). The probe can thus assist in the investigations of LAP-associated clinical disease.
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http://dx.doi.org/10.1021/acs.analchem.2c04587 | DOI Listing |
Br J Ophthalmol
December 2024
Department of Ophthalmology and Medical Research Center, Oulu University Hospital; Research Unit of Clinical Medicine, University of Oulu, Oulu, Finland.
Background/aims: The purpose of this study is to define genetic factors associated with anterior uveitis through genome-wide association study (GWAS).
Methods: In this GWAS meta-analysis, we combined data from the FinnGen, Estonian Biobank and UK Biobank with a total of 12 205 anterior uveitis cases and 917 145 controls. We performed a phenome-wide association study (PheWAS) to investigate associations across phenotypes and traits.
Ying Yong Sheng Tai Xue Bao
October 2024
State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau, Institute of Soil and Water Conservation, Northwest A&F University, Yangling 712100, Shaanxi, China.
Understanding the impacts of freeze-thaw action on soil microbial nutrient limitation can provide important support for sustainable utilization of black soil resources. We analyzed the impacts of freeze-thaw action on soil microbial nutrient limitation on a slope farmland located in a typical thick Mollisol region of Keshan County, Heilongjiang Province. We examined the responses of soil microbial nutrient limitation to soil erosion rates through measuring soil nutrient, soil microbial biomass, and soil enzyme activities before and after freeze-thaw under natural conditions, and estimated the soil erosion rates by Cs tracing technology.
View Article and Find Full Text PDFUnlabelled: Proteolysis of hydrophobic helices is required for complete breakdown of every transmembrane protein trafficked to the lysosome and sustains high rates of endocytosis. However, the lysosomal mechanisms for degrading hydrophobic domains remain unknown. Combining lysosomal proteomics with functional genomic data mining, we identify Lysosomal Leucine Aminopeptidase (LyLAP; formerly Phospholipase B Domain-Containing 1) as the hydrolase most tightly associated with elevated endocytic activity.
View Article and Find Full Text PDFAMB Express
December 2024
College of Life Science, South-Central Minzu University, Wuhan, 430074, China.
Amp0279 (EC 3.4.11.
View Article and Find Full Text PDFJ Hazard Mater
November 2024
Grasslands and Sustainable Farming, Production Systems Unit, Natural Resources Institute Finland, Halolantie 31A, Maaninka, Kuopio FI-71750, Finland. Electronic address:
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