A broad host range (BHR)-inducible T7 RNA polymerase system was developed, enabling induction with isopropyl-β-d-thiogalactopyranoside (IPTG), similar to the Escherichia coli strain BL21(DE3) protocol, but it is now applicable in a wide range of bacteria. This system allows for high protein yields and purification from diverse Gram-negative bacteria, including the native host.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933640 | PMC |
| http://dx.doi.org/10.1128/mra.01119-22 | DOI Listing |
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