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| http://dx.doi.org/10.1177/00185787221125721 | DOI Listing |
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A yeast-based reverse genetics system to generate HCoV-OC43 reporter viruses encoding an eighth subgenomic RNA.
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Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada.
Unlabelled: Coronaviruses have large, positive-sense single-stranded RNA genomes that challenge conventional strategies for mutagenesis. Yeast genetics has been used to manipulate large viral genomes, including those of herpesviruses and coronaviruses. This method, known as transformation-associated recombination (TAR), involves assembling complete viral genomes from dsDNA copies of viral genome fragments via homologous recombination in .
View Article and Find Full Text PDFDysregulation of lung epithelial cell homeostasis and immunity contributes to Middle East respiratory syndrome coronavirus disease severity.
mSphere
January 2025
Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Coronaviruses (CoV) emerge suddenly from animal reservoirs to cause novel diseases in new hosts. Discovered in 2012, the Middle East respiratory syndrome coronavirus (MERS-CoV) is endemic in camels in the Middle East and is continually causing local outbreaks and epidemics. While all three newly emerging human CoVs from the past 20 years (SARS-CoV, SARS-CoV-2, and MERS-CoV) cause respiratory disease, each CoV has unique host interactions that drive differential pathogeneses.
View Article and Find Full Text PDFLong COVID-19-related and non-COVID-19 postviral olfactory dysfunction a comparative MRI study focusing on the olfactory cleft and bulbs.
Front Neurol
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Graduate School, Beijing University of Chinese Medicine, Beijing, China.
Objective: To compare the magnetic resonance imaging (MRI) features of the olfactory cleft (OC) and olfactory bulbs (OBs) in patients with long COVID-19-related (LCOD) and non-COVID-19 postviral olfactory dysfunction (NCPVOD) to explore mechanisms underlying persistent olfactory dysfunction.
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Population dynamics and digitalization: implications for COVID-19 data sources in South Africa-a scoping review.
Front Public Health
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Faculty of Humanities, North-West University, Mafikeng, South Africa.
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Westat, Rockville, Maryland (Ghose, Beehler, Crocker, Hoey, Masiakowski, Karakus); National Association of State Mental Health Program Directors Research Institute, Falls Church, Virginia, and Department of Psychiatry, University of Michigan, Ann Arbor (Pinals); Department of Psychiatry, University of Maryland, College Park (Goldman); Center for Mental Health Services (Thomas) and Office of the Assistant Secretary for Mental Health and Substance Use (Patel), Substance Abuse and Mental Health Services Administration (SAMHSA), Rockville, Maryland.
Objective: Youth inpatient and residential treatment psychiatric services are essential components of the continuum of care. Concern has grown about the diminished availability of these services and the increasing need for them. This study aimed to examine the number of youths treated at inpatient and residential psychiatric facilities over a 12-year period and to assess the perceptions of state mental health authorities (SMHAs) about the reasons for changes in availability.
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