AI Article Synopsis
- The study investigates the effects of polysaccharides from a traditional herbal medicine on healing intestinal mucosal injuries, focusing on calcium signaling mechanisms.
- Using a rat model and various laboratory techniques, researchers found that the herbal polysaccharides helped reduce mucosal damage and promoted cell migration, which is vital for recovery.
- Results indicate that the polysaccharides increase calcium levels in the intestines and enhance the expression of calcium-regulating proteins, effectively aiding in the repair of intestinal injury.
Article Abstract
Background And Aim: , a key herbal medicine of replenishing Qi and tonifying Spleen, is widely used in the treatment of gastrointestinal diseases in East Asia. In this study, we aim to investigate the potential effects and mechanisms of polysaccharides from (PGP) on intestinal mucosal restitution which is one of the crucial repair modalities during the recovery of mucosal injury controlled by the Ca signaling.
Methods: Rat model of intestinal mucosal injury was induced by indomethacin. The fractional cell migration was carried out by immunohistochemistry staining with BrdU. The morphological observations on intestinal mucosal injury were also performed. Intestinal epithelial cell (IEC-6) migration in vitro was conducted by scratch method. Western-blot was adopted to determine the expressions of PLC-γ1, Rac1, TRPC1, RhoA and Cav-1. Immunoprecipitation was used to evaluate the levels of Rac1/PLC-γ1, RhoA/TRPC1 and Cav-1/TRPC1.
Results: The results showed that PGP effectively reduced the assessment of intestinal mucosal injury, reversed the inhibition of epithelial cell migration induced by Indomethacin, and increased the level of Ca in intestinal mucosa in vivo. Moreover, PGP dramatically promoted IEC-6 cell migration, the expression of Ca regulators (PLC-γ1, Rac1, TRPC1, Cav-1 and RhoA) as well as protein complexes (Rac1/PLC-γ1, Cav-1/TRPC1 and RhoA/TRPC1) in vitro.
Conclusion: PGP increases the Ca content in intestinal mucosa partly through controlling the regulators of Ca mobilization, subsequently promotes intestinal epithelial cell migration, and then prevents intestinal mucosal injury induced by indomethacin.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9834020 | PMC |
| http://dx.doi.org/10.1016/j.jgr.2022.05.010 | DOI Listing |
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