Protocol for standardized intrathymic injection in mice.

STAR Protoc

Institute of Microbiology and Immunology, National Yang Ming Chiao Tung University, Taipei 112, Taiwan; Taiwan International Graduate Program in Molecular Medicine, National Yang Ming Chiao Tung University and Academia Sinica, Taipei, Taiwan. Electronic address:

Published: March 2023

AI Article Synopsis

  • Current intrathymic injection methods often lead to complications and have challenges in accessing the necessary equipment.
  • This study introduces a standardized intrathymic injection protocol that is minimally invasive and only requires basic tools.
  • It outlines how to locate injection sites for effective drug delivery and demonstrates the success of the procedure by using Indian ink in the injection solution.

Article Abstract

Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2022)..

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9845999PMC
http://dx.doi.org/10.1016/j.xpro.2022.102010DOI Listing

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Protocol for standardized intrathymic injection in mice.

STAR Protoc

March 2023

Institute of Microbiology and Immunology, National Yang Ming Chiao Tung University, Taipei 112, Taiwan; Taiwan International Graduate Program in Molecular Medicine, National Yang Ming Chiao Tung University and Academia Sinica, Taipei, Taiwan. Electronic address:

Article Synopsis
  • Current intrathymic injection methods often lead to complications and have challenges in accessing the necessary equipment.
  • This study introduces a standardized intrathymic injection protocol that is minimally invasive and only requires basic tools.
  • It outlines how to locate injection sites for effective drug delivery and demonstrates the success of the procedure by using Indian ink in the injection solution.
View Article and Find Full Text PDF

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