Optimization and adsorption characteristics of beads based on heat-inactivated bacterial biomaterial towards the pesticide Cypermethrin.

Aims: Beads containing heat-inactivated bacterial biomaterial (BBBs) were prepared for removal of cypermethrin (CPM) and the conditions for this removal were evaluated and optimized via single-factor coupled orthogonal experiments based on five factors. The adsorption characteristics of BBBs and the binding mechanism were then explored.

Methods And Results: Results showed that the adsorption rate of CPM could reach 98% with beads prepared under optimized conditions: equal volumes of Lactobacillus cell debris derived from 1×1011 CFU; 2% hydroxypropyl-β-cyclodextrin and 2.5% activated carbon concentration, were mixed to give mixture TM, and this and SA, was mixed 1:4 with sodium alginate (SA) and beads were prepared using a 26-Gauge needle). The best adsorption conditions were initial CPM concentration of 10 mg l-1, incubation time of 24 h, and rotational speed of 180 rpm. BBBs have a well-formed structure and abundant surface functional groups, such as -COOH, -OH, -NH, -CH, -CO, -C=C. The adsorption process conformed to pseudo-second-order kinetic, and it was also a Freundlich monolayer adsorption, and the calculated maximum adsorption capacity was 9.69 mg g-1 under optimized conditions.

Conclusions: BBBs showed the highest CPM removal capacity and a good tolerance ability.

Significance And Impact Of The Study: Our results provided a theoretical foundation for developing an adsorbent with heat-inactivated Lactobacillus plantarum (L. plantarum) RS60 for removing CPM in wastewater or drinks.